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引用次数: 2
摘要
杨志强、陈志强、陈志强，2010。甘蓝(Brassica oleracea var. acephala) SSR标记辅助选择的评价Bulg。j·阿格利司。科学。本研究的目的是检测与甲基磺胺基烷基硫代葡萄糖苷相关的SSR标记Ol12FO2及其在甘蓝(Brassica oleracea var. acephala)标记辅助选择(MAS)中的潜在应用。甲基磺胺烷基硫代葡萄糖苷，如糖苷和葡萄糖苷，已知具有促进健康和预防各种癌症的特性。与这些重要性状相关的标记对于加快育种计划是很重要的，这些育种计划旨在选择所需性状以进一步用于产生改良的新品系。本研究结果表明，利用SSR标记Ol12FO2成功扩增了羽衣甘蓝基因型的基因组DNA，获得了预期大小的条带。结果表明，Ol12FO2在甘蓝基因型中具有多态性。本研究的结果表明，在不同的蔬菜芸苔属中，标记的扩增是成功的。
EVALUATION OF AN SSR MARKER FOR MARKER-ASSISTED SELECTION IN KALE (BRASSICA OLERACEA var. ACEPHALA)
SARIKAMIS, G., R. YANMAZ, A. BALKAYA and B. BELGE, 2010. Evaluation of an SSR marker for marker-assisted selection in kale (Brassica oleracea var. acephala). Bulg. J. Agric. Sci., 16: 36-41 The objective of the current study is to test an SSR marker (Ol12FO2) associated with methylsulphinylalkyl glucosinolates and its potential application for marker assisted selection (MAS) in kale (Brassica oleracea var. acephala). Methylsulphinylalkyl glucosinolates such as glucoiberin and glucoraphanin are known to have health promoting properties and to protect against various forms of cancer. Markers associated with such important traits are important to expedite breeding programs aimed to select for desired traits for further use in generating improved novel lines. The findings of the present study revealed that successful amplifications with genomic DNA from kale genotypes were observed with the SSR marker Ol12FO2 and bands of expected sizes were obtained. It was determined that Ol12FO2 was polymorphic among kale genotypes. The results obtained in the present study demonstrated successful amplification of markers within different vegetable brassicas.
期刊介绍：
Bulgarian Journal of Agricultural Science (BJAS) is a peer-reviewed, open access, online and print journal, issued bimonthly. It has been published in English since 1995. It publishes original research papers, review articles and brief communications from all areas of agricultural science including agricultural economics, agroecology, agrochemisty and soil science, crop science, plant protection, animal science, veterinary medicine, fisheries and aquaculture, agricultural engineering, food technologies and other related areas.
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