亚硝酸盐诱导的阿尔茨海默型痴呆大鼠脑微循环床血管内皮形态功能状态的动态变化及间充质干细胞的应用

Q4 Medicine Medicinski Casopis Pub Date : 2021-01-01 DOI:10.5937/mckg55-31775
Y. Zorenko, G. Gubina-Vakulyck, O. Pavlova, T. Gorbach, E. Shchegelskaya, E. Omelchenko
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引用次数: 2

摘要

目标。本研究的目的是评估亚硝酸盐诱导的阿尔茨海默型痴呆大鼠脑微循环床血管内皮形态功能状态。方法:在实验结束后14 d,采用酶免疫法和光度法测定亚硝酸盐诱导痴呆模型大鼠(亚硝酸钠腹腔注射14和28 d)血清中内皮素-1、VEGF-A、eNOS和血管性血癌因子的含量。采用Einarson染色法和免疫组织化学染色法对脑组织切片进行VEGF抗体分期染色。结果。随着亚硝酸钠给药时间的延长,大鼠脑毛细血管和神经元损伤程度加重,“存活”神经元出现营养不良,产生VEGF的能力下降。在未给药MSCs的组中,经过14天的“再生期”后,内皮细胞、皮层和海马神经元进一步不同程度地刺激VEGF的产生。在引入干细胞的组中,毛细血管数量增加,在某些情况下出现内皮增生。结论。在亚硝酸盐诱发痴呆的动物中,注意到对毛细血管床内皮的剂量依赖性损伤。从损伤第一天起,VEGF表达即可证明血管再生。干细胞管理更有效地刺激毛细血管再生,证明了显著增加的脑毛细血管的数量。
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Dynamics of indicators of the endothelium morphofunctional state of the brain microcirculatory bed vessels in rats with nitrite-induced Alzheimer's type dementia on the background of mesenchymal stem cell administration
Objective. The aim of this study was to assess the vascular endothelium morphofunctional state of the brain microcirculatory bed in rats with nitrite-induced Alzheimer's type dementia on the background of stem cells administration. Methods. 14 days after the experiment's end, the endothelin-1, VEGF-A, eNOS, von Willebrand factor were determined in blood serum by the enzyme immunoassay and photometric methods in rats with a model of nitrite-induced dementia (14 and 28 days of sodium nitrite intraperitoneal introduction) with and without mesenchymal stem cells (MSCs) administration. The brain slices were stained according to the Einarson's method and immunohistochemically by staging the reaction with antibodies to VEGF. Results. With an increase in the sodium nitrite administration period, the degree of damage of brain capillaries and neurons increased, dystrophy of "surviving" neurons developed and ability to produce VEGF decreased. After 14 days of "regeneration period" in groups without MSCs administration, further stimulation of VEGF production by endotheliocytes, cortex and hippocampus neurons of varying degrees was observed. In groups where stem cells were introduced, the number of capillaries increased, with endothelial hyperplasia in some cases. Conclusion. In animals with nitrite-induced dementia, dose-dependent damage to the endothelium of the capillary bed is noted. From the first day damage the vascular regeneration can be proved by VEGF expression. The stem cells administration more effectively stimulates capillary regeneration, as evidenced by a noticeable increase of the number of brain capillaries.
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Medicinski Casopis
Medicinski Casopis Medicine-Medicine (all)
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