Dark and Photoinduced Cytotoxicity of Cationic Chlorin e6 Derivatives with Different Numbers of Charged Groups

Dark and photoinduced toxicity of several novel and previously described derivatives of chlorin e 6 with tetraalkylammonium substituents differing from each other in number of cationic groups and their location in the macrocycle were studied in HeLa cells and mammalian erythrocytes. The optimal design of cationic derivatives of chlorin e 6 that provides the most efficient photodynamic effect was shown to require the localization of cationic substituents in the same part of the molecule. In combination with the hydrophobicity of the chlorin macrocycle, it increases the amphiphilic properties of the whole molecule and enhances its interaction with biomembranes. incubated 3 h thermostatic shaker corresponding volume of 0.2 % DMSO. The of pho activity inferred based on the degree of erythrocyte hemolysis after 1 and 3 h of incubation at constant irradiation of cells with the red light at wavelength of 660 nm (light source LEDs with a power of 60 mW each). The of cytotox inferred on the degree of hemolysis after 1 and 3 h of incubation of erythrocytes in the