微生物引物：转座子定向插入位点测序（TraDIS）：一种连接基因型和表型的高通量方法。

IF 2.6 4区生物学 Q3 MICROBIOLOGY Microbiology-Sgm Pub Date : 2023-11-01 DOI:10.1099/mic.0.001385

Isabel A Warner, Weine J Kok, Nicole Martinelli, Zihao Yang, Emily C A Goodall, Ian Henderson

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引用次数: 0

摘要

基因筛选是连接表型和基因型的关键工具。转座子诱变是最早将遗传位点与表型联系起来的遗传方法之一。下一代测序的出现改变了这项技术的使用，允许快速询问整个基因组中与表型相关的基因。一种方法是转座子定向插入位点测序（TraDIS）。在这里，我们描述了该方法、技术的最新发展，以及与其他基因筛查工具相比，该方法的优点和缺点。

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Microbial Primer: Transposon directed insertion site sequencing (TraDIS): A high throughput method for linking genotype to phenotype.

Genetic screens are a key tool for linking phenotype and genotype. Transposon mutagenesis was one of the first genetic methodologies to associate genetic loci with phenotypes. The advent of next-generation sequencing transformed the use of this technique allowing rapid interrogation of whole genomes for genes that correlate with phenotype. One method is transposon directed insertion-site sequencing (TraDIS). Here we describe the method, recent developments in technology, and the advantages and disadvantages of this method compared to other genetic screening tools.

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来源期刊

Microbiology-Sgm 生物-微生物学

CiteScore

4.60

自引率

7.10%

发文量

132

审稿时长

3.0 months

期刊介绍： We publish high-quality original research on bacteria, fungi, protists, archaea, algae, parasites and other microscopic life forms. Topics include but are not limited to: Antimicrobials and antimicrobial resistance Bacteriology and parasitology Biochemistry and biophysics Biofilms and biological systems Biotechnology and bioremediation Cell biology and signalling Chemical biology Cross-disciplinary work Ecology and environmental microbiology Food microbiology Genetics Host–microbe interactions Microbial methods and techniques Microscopy and imaging Omics, including genomics, proteomics and metabolomics Physiology and metabolism Systems biology and synthetic biology The microbiome.