含有GGDEF结构域的蛋白质影响霍乱弧菌的运动和生物膜的形成，并受Fur和HapR的负调控。

IF 3 3区医学 Q2 ENVIRONMENTAL SCIENCES Biomedical and Environmental Sciences Pub Date : 2023-10-01 DOI:10.3967/bes2023.080

He GAO , Li Zhi MA , Qin QIN , Yao CUI , Xiao Han MA , Yi Quan ZHANG , Biao KAN

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引用次数: 0

摘要

目的：研究VCA0560基因是否在霍乱弧菌中起活性二鸟苷酸环化酶（DGC）的作用，以及Fur和HapR对其转录的调控作用，和游泳运动测定。通过发光法、电泳迁移率偏移法和DNA酶I足迹法分析了Fur和HapR对VCA0560基因的调控。结果：VCA0560基因突变不影响霍乱弧菌生物膜的形成、运动和c-di-GMP的合成，其过表达显著增强了生物膜的生成和细胞内c-di-GMP-水平，但降低了运动能力。Fur和主要群体感应调节因子HapR直接抑制VCA0560基因的转录。结论：过度表达的VCA0560在霍乱弧菌中具有活性DGC的作用，其转录受到Fur和HapR的抑制。

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Protein Containing the GGDEF Domain Affects Motility and Biofilm Formation in Vibrio cholerae and is Negatively Regulated by Fur and HapR

Objective

This study aimed to investigate whether the VCA0560 gene acts as an active diguanylate cyclase (DGC) in Vibrio cholerae and how its transcription is regulated by Fur and HapR.

Methods

The roles of VCA0560 was investigated by utilizing various phenotypic assays, including colony morphological characterization, crystal violet staining, Cyclic di-GMP (c-di-GMP) quantification, and swimming motility assay. The regulation of the VCA0560 gene by Fur and HapR was analyzed by luminescence assay, electrophoretic mobility shift assay, and DNase I footprinting.

Results

VCA0560 gene mutation did not affect biofilm formation, motility, and c-di-GMP synthesis in V. cholerae, and its overexpression remarkably enhanced biofilm formation and intracellular c-di-GMP level but reduced motility capacity. The transcription of the VCA0560 gene was directly repressed by Fur and the master quorum sensing regulator HapR.

Conclusion

Overexpressed VCA0560 functions as an active DGC in V. cholerae, and its transcription is repressed by Fur and HapR.

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来源期刊

Biomedical and Environmental Sciences 环境科学-公共卫生、环境卫生与职业卫生

CiteScore

2.60

自引率

8.60%

发文量

2170

审稿时长

1.0 months

期刊介绍： Biomedical and Environmental Sciences (BES) is a peer-reviewed journal jointly established by the Chinese Center for Disease Control and Prevention (China CDC) and the Coulston International Corporation (CIC), USA in 1988, and is published monthly by Elsevier. It is indexed by SCI, PubMed, and CA. Topics covered by BES include infectious disease prevention, chronic and non-communicable disease prevention, disease control based on preventive medicine, and public health theories. It also focuses on the health impacts of environmental factors in people''s daily lives and work, including air quality, occupational hazards, and radiation hazards. Article types considered for publication include original articles, letters to the editor, reviews, research highlights, and policy forum.