骨髓源性基质细胞系M210B4的表型和功能特征及其与原代骨髓基质细胞的比较

Shweta Singh, Suprita S. Ghode, M. Devi, L. Limaye, V. Kale
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引用次数: 3

摘要

骨髓基质细胞(BMSCs)或骨髓间充质基质细胞系组成的体外共培养系统为研究造血干细胞(hsc)的微环境或生态位调控提供了重要线索。在目前的研究中,我们比较了骨髓来源的间充质干细胞系M210B4与骨髓间充质干细胞的表型和功能特征。我们证明M210B4在表型特征方面与BMSCs相似。与BMSCs不同,M210B4仅向成脂谱系分化,难以向成骨谱系分化。然而,通过对共培养输出细胞的流式细胞术分析,M210B4细胞表现出更高的hsc支持能力。我们观察到,与BMSCs相比,M210B4细胞显示出更高的p44/42和p38 MAPK通路的激活,这有助于它们在体外提供更高的hsc支持。综上所述,M210B4形成了一个适合体外研究HSC调控的体系。
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Phenotypic and functional characterization of a marrow-derived stromal cell line, M210B4 and its comparison with primary marrow stromal cells
In vitro co-culture system consisting of bone marrow stromal cells (BMSCs) or mesenchymal stromal cell lines of marrow origin has provided important clues about the regulation of hematopoietic stem cells (HSCs) by their microenvironment or niche. In the current studies, we have compared phenotypic and functional characters of a marrow-derived mesenchymal stem cell line, M210B4, with BMSCs. We demonstrate that M210B4 resembles BMSCs in terms of phenotypic characters. Unlike the BMSCs, M210B4 differentiated only towards adipogenic lineage, and was refractory towards osteogenic differentiation. However, M210B4 cells exhibited a higher HSC-supportive ability as assessed by flow cytometry analyses of the output cells from co-cultures. We observed that M210B4 cells show a constitutively higher activation of p44/42 and p38 MAPK pathways compared to BMSCs, contributing to their higher HSC-support in vitro. Overall, the results show that M210B4 forms a suitable in vitro system to study HSC regulation in vitro.
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