共聚焦显微镜通过互易光纤图像检测

T. Wilson, F. Reinholz
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引用次数: 7

摘要

我们描述了一个紧凑形式的共聚焦扫描显微镜使用半导体激光器。共聚焦操作是通过使用单模光纤来保证发射到显微镜中的光和从物体收集信号。所收集的光被允许重新进入激光器,并且图像被检测为对来自激光功率监测二极管的信号的调制。将图像与传统点检测器得到的图像进行比较。发现互反方案的对准公差比传统的共焦系统大大降低。
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Confocal microscopy via reciprocal optical fibre image detection

We describe a compact form of confocal scanning microscope using a semiconductor laser. Confocal operation is ensured by the use of a single mode optical fibre for both launching the light into the microscope and collecting the signal from the object. The collected light is allowed to re-enter the laser and the image is detected as a modulation on the signal from the laser power monitor diode. Images are compared with those obtained from traditional point detectors. The alignment tolerances of the reciprocal scheme are found to be greatly reduced over conventional confocal systems.

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