Yali Chen , Zuhong Lu , Weiping Wang , Mei Zhou , Jianguo Wu
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A nonradioactive dot-blot hybridization for Epstain-Barr virus with probe generated using the polymerase chain reaction
A nonradioactive probe was utilized in dot-blot hybridization for the detection of infectious Epstein-Barr virus. A polymerase chain reaction PCR-generated oligonucleotide, a 121 bp segment was labeled with digoxigenin (DIG)-11-dUTP. The hybridization results were detected by using an alkaline phosphatase-conjugated antibody to DIG and the enzyme substrates. The DIG-EB DNA allowed the detection limit in 0.1 pg of DNA. The samples from fifteen patients with nasopharyneal cancer (NPC) were examined.
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