Mechanism of octreotide in acute necrotizing pancreatitis (ANP) of rats: An experimental study

OBJECTIVE: To study the action of octreotide in acute necrotizing pancreatitis (ANP) in rats. METHODS: The effect of octreotide on pancreatic acinar cells with respect to amino acid uptake, protein synthesis and secretion of enzymes was observed by using radioactive tracing and autoradiography on both healthy rats and rats with ANP. RESULTS: It was shown that octreotide has no effect on pancreatic acinar cells with respect to amino acid uptake and protein synthesis in both ANP and in control rats. However, octreotide was able to inhibit the secretion of enzyme-proteins. There were secretions from the basolateral membrane of the acinar cells in ANP rats, which could be reduced by treatment with octreotide. CONCLUSIONS: Octreotide has no effect on pancreatic acinar cells with respect to amino acid uptake and enzyme-protein synthesis, but it can inhibit the secretion of enzyme-proteins, in particular, from the basolateral membrane of ANP rats and prevent the accumulation of zymogen granules in the interstitium.