银纳米颗粒在肠杆菌中的合成及其对番茄枯萎菌的抑菌活性

M. El-Zahed, M. Nour El-Dein, M. Abou-Dobara, Z. Baka
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引用次数: 0

摘要

本研究对不同水源中细菌的分离进行了研究。在这些菌株中;肠杆菌表现出细胞外生物合成的银纳米颗粒具有良好的单分散性。肠杆菌在35°C的黑暗条件下,在72小时内产生了非常稳定的纳米颗粒。采用紫外-可见分光光度计、透射电子显微镜、Zeta电位分析仪和体积大小分布对纳米颗粒进行了表征。Enterobacter sp.合成了9.45-17.15 nm大小的球形银纳米颗粒。通过zeta大小的体积分布证实了稳定蛋白与纳米颗粒的存在和结合。我们的研究表明,银纳米颗粒是植物病原真菌番茄枯萎病的有效抑制剂。
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Biosynthesis of silver nanoparticles by Enterobacter sp. and their antifungal activity against the phytopathogenic fungus Fusarium oxysporum f. sp. lycopersici
This study was concerned with the isolation of bacterial strains from different water sources. Among these strains; Enterobacter sp. exhibited extracellularly biosynthesized silver nanoparticles with good monodispersity. Enterobacter sp. has produced extremely stable nanoparticles within 72 hrs at 35°C in dark conditions. These nanoparticles are characterized by UV–vis spectrophotometer, transmission electron microscopy, Zeta potential analyzer, and size distribution by volume. Enterobacter sp. synthesized 9.45-17.15 nm sized spherical shaped silver nanoparticles. The presence and binding of stabilized proteins with nanoparticles were confirmed by the zeta size distribution by volume. Our study demonstrates that silver nanoparticles are potent inhibitors of the plant pathogenic fungus, Fusarium oxysporum f. sp. lycopersici.
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