牛粪灰对玉米象甲的防治效果研究。(鞘翅目:象甲科)

M. Sulaiman
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摘要

研究了牛粪灰(CDA)对玉米籽粒对玉米象虫(Sitophilus zeamais Motsch)的保护作用。在28-32℃和60-75%相对湿度(R.H.)的实验室条件下。采用浓度为0.625、1.25、2.50、5.00、10.00% (w/w)的CDA,以浓度为0.625%的氯菊酯粉为阳性对照,在不添加氯菊酯和灰的培养皿中设置阴性对照。在每个培养皿中放入五对象鼻虫,并用细布覆盖。试验采用完全随机设计(CRD),重复3次。确定治疗对成人死亡率的影响。还对不同剂量CDA处理的种子成虫羽化和重量损失进行了评估。研究结果表明,不同剂量CDA对玉米玉米玉米的成虫死亡率差异显著(p < 0.05),处理后96 h (HAT)死亡率为13.33% ~ 100.00%。研究结果还表明,CDA处理后24 h对玉米玉米瘟病菌的LD50 (HAT)为4.57% (w/w)。在所有处理中均未发现玉米玉米螟F1成虫。最低剂量和对照处理的籽粒体重减轻差异显著(P<0.05)。结果表明,CDA对玉米发芽无不良影响。因此,有必要确定CDA中的生物活性化合物,这些活性化合物负责其对玉米玉米的杀虫能力。关键词:成虫羽化,牛粪灰(CDA),发芽量,死亡率,谷物危害,玉米象
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Studies on the Efficacy of Cow Dung Ash against Maize Weevil, Sitophilus zeamais Motsch. (Coleoptera: Curculionidae)
Experiments were carried out to assess the protectant ability of cow dung ash (CDA) to maize grains against Sitophilus zeamais Motsch. under laboratory conditions of 28-32oC and 60-75% relative humidity (R.H.). The CDA at different doses of 0.625, 1.25, 2.50, 5.00, 10.00% (w/w) were used and permethrin powder at 0.625% used as positive control, negative control was also set up in different Petri dishes to which no permethrin or ashes were added. Five pairs of weevils were introduced into each of the Petri dishes and covered with muslin cloth. The experiment was arranged in a completely randomized design (CRD) and replicated three times. Effect of the treatments on adult mortality was determined. Adult emergence and weight losses in seeds treated with CDA at different doses were also assessed. Findings of the study revealed that the adult mortality of S. zeamais among different CDA doses differed significantly (p < 0.05) and ranged from 13.33 to 100.00% at 96 hours after treatment (HAT). Results of the study also showed that LD50 of CDA against S. zeamais at 24 hours after treatment (HAT) was 4.57 % (w/w). There were no F1 adult emergences of S. zeamais in all the treatments. Weight losses were found to differ significantly (P<0.05) which were observed in grains treated with the lowest dose and the control only. It was found that CDA had no adverse effect on the germination of maize. There is therefore the need to identify the bioactive compounds in CDA that are responsible for its insecticidal ability against S. zeamais. Key words: Adult emergence, Cow dung ash (CDA), Germination capacity, Mortality, Grain damage, Sitophilus zeamais
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