荧光气相生物传感器(生物嗅探器),具有UV-LED发光,用于检测甲醛蒸气

Ye Ming, T. Gessei, K. Miyajima, Munkhbayar Munkhjargal, T. Arakawa, K. Mitsubayashi
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引用次数: 1

摘要

研制了一种用于气体甲醛(FA)脱毒的光纤生化气体传感器(生物嗅探器),并应用于FA脱毒的现场评价。该生物嗅探器通过甲醛脱氢酶反应产物烟酰胺腺嘌呤二核苷酸(NADH)的荧光检测FA。生物嗅探器对气态FA的检出限为2.5 ppb,足以用于评价FA的解毒作用。然后应用生物嗅探器测量N. E. " boston oniensis "对植物的修复作用。在95分钟内,测量室内的FA水平下降到检测极限(2.5 ppb),低于室内空气的FA水平(12 ppb),当室内通风时,该值上升到FA水平。
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Fluorometric gas-phase biosensor (bio-sniffer) with UV-LED emission light for formaldehyde vapor
A fibre-optic biochemical gas sensor (bio-sniffer) for gaseous formaldehyde (FA) was developed and applied for on-site assessment of FA detoxification. The bio-sniffer measures FA as a fluorescence of reduced nicotinamide adenine dinucleotide (NADH) which is the product of formaldehyde dehydrogenase reaction. The detection limit of the bio-sniffer for gaseous FA was 2.5 ppb, which is enough lower for the purpose of evaluating FA detoxification. The bio-sniffer was then applied to measure phytoremediation with N. E. “Bostoniensis”. The FA level in the measurement chamber decreased down to the detection limit (2.5 ppb), which is lower level than FA level of room air (12 ppb), within 95 min and the value increased to FA level when the chamber was vented.
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