Mangosteen Garcinia mangostana L. simplicia effect on bone structure and behaviour of Wader Fish Rasbora lateristriata (Bleeker, 1854) Embryo

Mangosteen (Garcinia mangostana L.) is a fruit originating from the tropics which belongs to the Clusiaceae family and has become a commodity with many enthusiasts from various countries. Previous research has shown that mangosteen peel extract has tremendous potential to be developed as an ingredient in herbal medicine. Mangosteen fruit contains anti-inflammatory, antibacterial, antifungal, antihistamine, antidiabetic, anticancer, and so on. The results of the phytochemical screening test of 95% ethanol extract on mangosteen rind showed that it contained flavonoid compounds, saponins, alkaloids, triterpenoids, tannins and polyphenols. There is no data on the effect of exposure to mangosteen pericarip simplicia, therefore it is necessary to study the effect of exposure to mangosteen peel decoction on animal models of Wader Pari Fish (Rasbora lateristriata), with parameters such as morphology and bone structure in larvae wader fish (Rasbora lateristriata). Alcian Blue Alizarin Red staining were used to study bone structure in wader fish (Rasbora lateristriata). Data were statistical analysis with SPSS ver. 25 and the significance was determined using one-way analysis of variance (ANOVA). The morphological examination results revealed that the embryo and larvae had no morphological abnormalities. The cranial cartilage examination also revealed that the larvae had no abnormalities, the length and the angle of the cranial cartilage did not change between the control and mangosteen pericarp simplicia treatment.