{"title":"雷帕霉素对TGF-β1诱导的LoVo结肠腺癌细胞上皮-间质转化的影响","authors":"Renhu Sun, Jiang Li, Jing Cui, Qing Lv, Xinghua Liu, Guobin Wang","doi":"10.1016/S1007-4376(09)60020-4","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p>To investigate the effect of Rapamycin on epithelial-mesenchymal transition(EMT) of LoVo colonic adenocarcinoma cells <em>in vitro</em>.</p></div><div><h3>Methods</h3><p>Cultured LoVo colonic adenocarcinoma cells were divided into three groups: negative control group, EMT-inducing group(TGF-β <sub>1</sub>) and EMT-interfering group(TGF-β <sub>1</sub> plus Rapamycin). E-cadherin expression in LoVo cells was detected by Western Blot, while the expression of vimentin was evaluated through immunocytochemistry. The Snail mRNA in LoVo cells was examined by RT-PCR.</p></div><div><h3>Results</h3><p>TGF-β <sub>1</sub> induced LoVo cell switching from polygonal to spindle-shaped. TGF-β <sub>1</sub> enhanced the expression of vimentin, but lowered the level of E-cadherin. In contrast, Rapamycin impaired the transition induced by TGF-β <sub>1</sub>. Rapamycin dramatically abrogated TGF-β <sub>1</sub>-induced vimentin expression and restored E-cadherin expression in LoVo cells. Rapamycin significantly repressed the up-regulation of Snail mRNA expression induced by TGF-β <sub>1</sub>.</p></div><div><h3>Conclusion</h3><p>Rapamycin dramatically abrogated TGF-β <sub>1</sub> induced Snail mRNA expression in LoVo cells, hence inhibiting EMT of these cells <em>in vitro</em>.</p></div>","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":"23 1","pages":"Pages 15-19"},"PeriodicalIF":0.0000,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1007-4376(09)60020-4","citationCount":"0","resultStr":"{\"title\":\"Effect of Rapamycin on TGF-β1-induced epithelial-mesenchymal transition in LoVo colonic adenocarcinoma cells\",\"authors\":\"Renhu Sun, Jiang Li, Jing Cui, Qing Lv, Xinghua Liu, Guobin Wang\",\"doi\":\"10.1016/S1007-4376(09)60020-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><p>To investigate the effect of Rapamycin on epithelial-mesenchymal transition(EMT) of LoVo colonic adenocarcinoma cells <em>in vitro</em>.</p></div><div><h3>Methods</h3><p>Cultured LoVo colonic adenocarcinoma cells were divided into three groups: negative control group, EMT-inducing group(TGF-β <sub>1</sub>) and EMT-interfering group(TGF-β <sub>1</sub> plus Rapamycin). E-cadherin expression in LoVo cells was detected by Western Blot, while the expression of vimentin was evaluated through immunocytochemistry. The Snail mRNA in LoVo cells was examined by RT-PCR.</p></div><div><h3>Results</h3><p>TGF-β <sub>1</sub> induced LoVo cell switching from polygonal to spindle-shaped. TGF-β <sub>1</sub> enhanced the expression of vimentin, but lowered the level of E-cadherin. In contrast, Rapamycin impaired the transition induced by TGF-β <sub>1</sub>. Rapamycin dramatically abrogated TGF-β <sub>1</sub>-induced vimentin expression and restored E-cadherin expression in LoVo cells. Rapamycin significantly repressed the up-regulation of Snail mRNA expression induced by TGF-β <sub>1</sub>.</p></div><div><h3>Conclusion</h3><p>Rapamycin dramatically abrogated TGF-β <sub>1</sub> induced Snail mRNA expression in LoVo cells, hence inhibiting EMT of these cells <em>in vitro</em>.</p></div>\",\"PeriodicalId\":100807,\"journal\":{\"name\":\"Journal of Nanjing Medical University\",\"volume\":\"23 1\",\"pages\":\"Pages 15-19\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2009-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S1007-4376(09)60020-4\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Nanjing Medical University\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1007437609600204\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Nanjing Medical University","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1007437609600204","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Effect of Rapamycin on TGF-β1-induced epithelial-mesenchymal transition in LoVo colonic adenocarcinoma cells
Objective
To investigate the effect of Rapamycin on epithelial-mesenchymal transition(EMT) of LoVo colonic adenocarcinoma cells in vitro.
Methods
Cultured LoVo colonic adenocarcinoma cells were divided into three groups: negative control group, EMT-inducing group(TGF-β 1) and EMT-interfering group(TGF-β 1 plus Rapamycin). E-cadherin expression in LoVo cells was detected by Western Blot, while the expression of vimentin was evaluated through immunocytochemistry. The Snail mRNA in LoVo cells was examined by RT-PCR.
Results
TGF-β 1 induced LoVo cell switching from polygonal to spindle-shaped. TGF-β 1 enhanced the expression of vimentin, but lowered the level of E-cadherin. In contrast, Rapamycin impaired the transition induced by TGF-β 1. Rapamycin dramatically abrogated TGF-β 1-induced vimentin expression and restored E-cadherin expression in LoVo cells. Rapamycin significantly repressed the up-regulation of Snail mRNA expression induced by TGF-β 1.
Conclusion
Rapamycin dramatically abrogated TGF-β 1 induced Snail mRNA expression in LoVo cells, hence inhibiting EMT of these cells in vitro.
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