Arı Spermasının Dondurma-Çözdürme Sonrası Spermatolojik Parametreleri Üzerine Metiyonin ve Sisteaminin Etkileri

sperma and (2.5 (P<0.05). Abstract In this study, it was aimed to determine the effects of methionine and cysteamine in the cryopreservation process of drone semen (Apis mellif-era). Semen samples were collected from sexually mature drones and pooled. The pooled semen was then divided into five equal volumes and diluted with different concentrations of methionine (2.5 and 5 mM), cysteamine (2.5 and 5 mM) and control. Post-thawed motility values; Methionine5, Cysteamine2,5 and Cysteamine5 groups had a possitive effect on compared to the the control group (P<0.05). It was observed that the antioxidant groups protected the plasma membrane integrity better than the control group (P<0.05). Acrosomal integrity rates was better in the methionine groups compared to the control group (P<0.05). Membrane lipid peroxidation was analyzed by measuring malondialdehyde (MDA) concentration post-thawed. It was observed that antioxidant-containing extenders had beneficial effects on drone semen parameters. Considering the semen parameters; The Methionine5 group was found to be the most effective group for freezing drone semen.