印度芥菜基因型品种筛选及抗疫病分子分析

Samriddhi Singh, N. Khan, S. Singh, H. Singh, D. Dwivedi
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摘要

印度芥菜[芥菜]Czern 8 # x38; # x38;是印度一种重要的食用油籽作物。在自然条件下筛选出抗白疫病的基因型154种。9个基因型(Rohini、PAB-14-14、PBZ-4、PRD-14-1、PAB-14-5、PRD-14-16、PAB- 14-17、phr2、Parvati)中度易感,29个易感,116个高度易感。在生化分析中,发现同一品种NDRS-2009-1的非侵染叶片和侵染叶片中,只有10个基因型的酚类值最高,而ndrs -1-11-1、Vaibhav的非侵染叶片和侵染叶片中,过氧化物酶活性最高。在过氧化氢酶活性方面,NDRS-2007、NDRS-2009-1和NDRS-2007、NDRS-2009-1均存在高度值。聚类分析树形图分为I和II两组,相似度均值为0.45,而I组只有一个品种NDRS-2009- 1。组II分为两个子群A和b,组A分为两个子群A1和A2,相似度为0.60,组A1分为两个子群A1-1和A1-2,子群A1-1中发现了三个品种NDRE-1-11-1, NDYR-2008和Narendra Rai,但NDRE-1-11-1 0.75更相似NDYR-2008和Narendra Rai。亚簇A1-2有两个品种Vaibhav和Rohini,两者相似度仅为0.70。A2组共发现NDRE -7、Vardan和PM-26 3个品种,但NDRE -7、Vardan差异均为0.81,PM-26和B组仅发现NDRS-2007 1个品种。
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Varietal screening and molecular analysis against alternaria blight in Indian mustard genotypes
Indian mustard [Brassica juncea (L.) Czern 8#x38;#x38; Coss] is an important edible oilseed crop in India. There are 154 genotypes screened under natural condition against Alternaria blight. Disease appeared on leaves after 90 days were noted as, 9 genotypes found moderately susceptible (Rohini, PAB-14-14, PBZ-4, PRD-14-1, PAB-14-5, PRD-14-16, PAB- 14-17, PHR-2, Parvati), 29 were susceptible and 116 were highly susceptible. In case of biochemical analysis, only ten genotypes, maximum phenolic value in non-infected and infected leaves was in found same variety NDRS-2009-1 and peroxidase activity maximum values was found non-infected and infected leaves NDRE-1-11-1, Vaibhav. In catalase activity, a height value was found non-infected and infected leaves varieties NDRS-2007, NDRS-2009-1. Cluster analysis dendogram is divided in two group I and II, these similarity showing mean 0.45 and group I had only one variety namely NDRS-2009- 1. Group II had divided in to two sub groups A and B. Group A is in to two clusters A1 and A2 in this group similarly 0.60 and cluster A1 divided in to two sub cluster A1-1 and A1-2, sub cluster A1-1 had found three varieties NDRE-1-11-1, NDYR-2008 and Narendra Rai but NDRE-1-11-1 0.75 more similar both varieties NDYR-2008 and Narendra Rai. Sub cluster A1-2 had two varieties Vaibhav and Rohini both similar only 0.70. Cluster A2 had found three varieties NDRE -7, Vardan and PM-26 but both NDRE -7, Vardan dissimilar 0.81 then PM-26 and group B had found only one variety NDRS-2007.
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