Withania Somnifera (L.)根提取物对过氧化氢诱导PC12的影响

R. Kumar, Rajat Dhariwal, Anurag Kumar, Mizuki Minata
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摘要

它也被用作一种原始药物,主要用于各种神经和免疫疾病。在印度地区,它也被称为Ashwagandha和“印度人参”。目前还缺乏关于香草根对HO-和Ab(1-42)诱导的细胞毒性的可能的神经保护特性的信息,这两种细胞毒性现在是针对痴呆症,特别是阿尔茨海默氏型痴呆症(AD)的创新治疗的目标。本研究在大鼠嗜铬细胞瘤PC12细胞分化的条件下,通过MTT法制备了对Ab-聚集纤维和HO细胞毒性具有保护作用的苦参干根水提物。结果表明,在浓度依赖性的情况下,乌苏根水提液预处理分化的PC12细胞对H2O2-和Ab(1-42)诱导的PC12细胞毒性均有较强的保护作用。用液相色谱-串联质谱法检测了乌龙花提取物,以研究可能导致所见效果的物质。大量发现了Withanolide衍生物,特别是withaferin A。这些发现支持了一种假说,即睡眠草可能在民族药理学上用于治疗氧化应激相关的认知疾病和其他神经退行性疾病。它们还显示了苦参根水提取物的神经保护活性。
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Effect of Withania Somnifera (L.) root extract\'s on PC12 induced by hydrogen peroxide, study
The L. is also used as a crude medicament that mostly effected in various neurological and immunological disorders. It is also known as Ashwagandha and “Indian ginseng” in Indian region. There is a lack of information on the possible neuroprotective properties of W. somnifera root against HO- and Ab(1-42)-induced cytotoxicity, which are now targeted for innovative treatments for dementia, particularly dementia of the Alzheimer's type (AD). According to this research, we prepared an aqueous extract of dried roots of W. somnifera that possess the protective effect against Ab- aggregated fibril and HO cytotoxicity through MTT assay with the help of differentiated rat pheochromocytoma PC12 cell. The findings indicate that, in a concentration-dependent manner, pretreatment of differentiated PC12 cells with aqueous extracts of W. somnifera root strongly protects differentiated PC12 cells against both H2O2- and Ab(1-42)-induced cytotoxicity. The W. somnifera extract was examined using liquid chromatography-serial mass spectrometry in order to look into the substances that could be responsible for the effects that were seen. Withanolide derivatives, notably withaferin A, were found in abundance. These findings support the hypothesis that W. somnifera may be used ethnopharmacologically to treat oxidative stress-related cognitive and other neurodegenerative illnesses. They also show the neuroprotective activities of an aqueous extract of W. somnifera root.
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