SARS-CoV - 2感染后骨关节炎患者滑液中蛋白质的氧化修饰

Kateryna Dvorshchenko, S. Borodin
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引用次数: 1

摘要

由严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)引起的2019冠状病毒病(COVID-19)大流行已在全球范围内构成严重的公共卫生威胁。它造成了许多并发症和死亡,因此在越来越多的国家,数百万人处于危险之中。一个特殊的群体包括慢性病患者,尤其是骨关节炎患者。众所周知,骨关节炎的发展与氧化应激和自由基的过量产生有关。蛋白质对自由基氧化非常敏感,因此它们的氧化水平反映了体内的氧化-抗氧化平衡。这项工作的目的是测定SARS-CoV2感染后骨关节炎患者滑液中蛋白质和巯基氧化修饰产物的浓度。所有的研究参与者被分成两组。第一组(n=22)为II-III度膝关节骨关节炎患者。第二组(n=14)是膝关节II-III度骨关节炎患者,他们在6-9个月前患有轻中度COVID-19。在进行的研究中,评估了所有实验组患者滑液中蛋白质氧化的产物。在与2,4-二硝基苯肼的反应中,通过检测羰基衍生物的含量来确定蛋白质氧化修饰产物的浓度。用Ellman法测定总巯基、蛋白结合巯基和非蛋白巯基(SH)的浓度。使用普遍接受的变分统计方法对研究结果进行处理。我们发现,在感染COVID-19的膝关节骨性关节炎患者的滑液中,蛋白质氧化修饰产物的浓度升高。在相同的实验条件下,巯基的浓度降低。检测到的变化表明,SARS-CoV-2感染后骨关节炎患者滑液中氧化-抗氧化平衡被破坏。这表明自由基过程的普遍存在和氧化应激的发展直接在关节中。我们的研究结果表明,骨关节炎患者在感染COVID-19后可能会发生更严重的关节损伤。
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OXIDATIVE MODIFICATION OF PROTEINS IN THE SYNOVIAL FLUID OF PATIENTS WITH OSTEOARTHRITIS AFTER SARS-CoV 2-INFECTION
The pandemic of coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has made a serious public health threat worldwide. It has caused many complications and deaths, so millions of people are at risk in more and more countries. A special group consists of patients with chronic diseases, particularly osteoarthritis. It is known that the development of osteoarthritis is associated with oxidative stress and excessive production of free radicals. Proteins are highly sensitive to free radical oxidation, so their oxidation level reflects the oxidant-antioxidant balance in the body. The aim of the work was to determine the concentration of products of oxidative modification of proteins and sulfhydryl groups in the synovial fluid of patients with osteoarthritis after SARS-CoV2 infection. All study participants were divided into two groups. The first group (n=22) is patients with osteoarthritis of the knee joints II-III degree. The second group (n=14) is patients with osteoarthritis of the knee joints II-III degree, who suffered a mild and moderate form of COVID-19 6-9 months ago. In the conducted studies, the products of protein oxidation in the synovial fluid of patients of all experimental groups were evaluated. The concentration of products of oxidative modification of proteins was determined by the level of carbonyl derivatives, which are detected in the reaction with 2,4-dinitrophenylhydrazine. The concentration of total, protein-bound and non-protein sulfhydryl (SH) groups was measured by the Ellman method. Processing of research results was carried out using generally accepted methods of variational statistics. It was established that in the synovial fluid of patients with osteoarthritis of the knee joints who contracted COVID-19, the concentration of products of oxidative modification of proteins increased. Under the same experimental conditions, the concentration of sulfhydryl groups decreased. The detected changes indicate a violation of the oxidant-antioxidant balance in the synovial fluid of patients with osteoarthritis after SARS-CoV-2 infection. This indicates the prevalence of free radical processes and the development of oxidative stress directly in the joint. Our results indicate that patients with osteoarthritis may develop more severe joint damage after infection with COVID-19.
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