肽YY刺激Caco-2肠细胞载脂蛋白A-IV基因的表达。

K. Sonoyama, K. Suzuki, T. Kasai
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引用次数: 7

摘要

采用半定量RT-PCR +内寡核苷酸探针Southern杂交技术研究胃肠激素YY肽对小肠上皮细胞系Caco-2载脂蛋白a - iv基因表达的影响。载脂蛋白a - iv mRNA水平随YY肽剂量和时间的变化而升高。Western blotting结果显示外源肽YY使细胞内载脂蛋白A-IV浓度升高。相比之下,载脂蛋白A-I、B和C-III mRNA对肽YY没有反应。分化后的Caco-2细胞表达Y1-而不表达Y2-和y5受体亚型mRNA。目前的研究结果表明,YY肽可能通过肠上皮细胞中y1受体亚型调节载脂蛋白A-IV基因的表达。
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Peptide YY stimulates the expression of apolipoprotein A-IV gene in Caco-2 intestinal cells.
The effect of peptide YY, a gastrointestinal hormone, on the expression of the apolipoprotein A-IV gene in the intestinal epithelial cell line Caco-2 was examined by semiquantitative RT-PCR followed by Southern hybridization with an inner oligonucleotide probe. Apolipoprotein A-IV mRNA levels were increased in response to peptide YY in a dose- and time-dependent fashion. Western blotting revealed that the exogenous peptide YY increased the intracellular concentration of apolipoprotein A-IV. In contrast, apolipoprotein A-I, B, and C-III mRNA did not respond to peptide YY. Differentiated Caco-2 cells expressed Y1- but not Y2- and Y5-receptor subtype mRNA. The present results suggest that peptide YY modulates apolipoprotein A-IV gene expression, likely via the Y1-receptor subtype in intestinal epithelial cells.
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