{"title":"利用maldi-tof / tof质谱法对全细胞加热法的优势蛋白进行评价","authors":"S. Ningrum, I. Wibawan","doi":"10.15414/JMBFS.3807","DOIUrl":null,"url":null,"abstract":"Immunoglobulin G (IgG) are the essential tools in diagnostic kits. The whole-cell is still considered to be used for animal‘s immunization to produce IgG. The goal of this study was to determine dominant protein on the Shiga toxin-producing Escherichia coli (STEC) O157:H7-induced typical antibody response also determined by MALDI-TOF/TOF mass spectrometry. The heating method was conducted at 71 °C and 83 °C for antigen preparation. The proteins were characterized using SDS-PAGE and evaluated by MALDI-TOF/TOF mass spectrometry. The present study reported that the dominant polyclonal antibody inducing protein was ompC E. coli. The evaluation of mass spectra by MALDI-TOF/TOF might help to identify the dominant polyclonal antibody inducing protein in future preparation of diagnostic kits.","PeriodicalId":22746,"journal":{"name":"The Journal of Microbiology, Biotechnology and Food Sciences","volume":"46 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"EVALUATION OF THE DOMINANT PROTEIN FROM WHOLE-CELL HEATING METHOD USING MALDI-TOF/TOF MASS SPECTROMETRY\",\"authors\":\"S. Ningrum, I. Wibawan\",\"doi\":\"10.15414/JMBFS.3807\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Immunoglobulin G (IgG) are the essential tools in diagnostic kits. The whole-cell is still considered to be used for animal‘s immunization to produce IgG. The goal of this study was to determine dominant protein on the Shiga toxin-producing Escherichia coli (STEC) O157:H7-induced typical antibody response also determined by MALDI-TOF/TOF mass spectrometry. The heating method was conducted at 71 °C and 83 °C for antigen preparation. The proteins were characterized using SDS-PAGE and evaluated by MALDI-TOF/TOF mass spectrometry. The present study reported that the dominant polyclonal antibody inducing protein was ompC E. coli. The evaluation of mass spectra by MALDI-TOF/TOF might help to identify the dominant polyclonal antibody inducing protein in future preparation of diagnostic kits.\",\"PeriodicalId\":22746,\"journal\":{\"name\":\"The Journal of Microbiology, Biotechnology and Food Sciences\",\"volume\":\"46 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-04-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Journal of Microbiology, Biotechnology and Food Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.15414/JMBFS.3807\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Journal of Microbiology, Biotechnology and Food Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15414/JMBFS.3807","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
EVALUATION OF THE DOMINANT PROTEIN FROM WHOLE-CELL HEATING METHOD USING MALDI-TOF/TOF MASS SPECTROMETRY
Immunoglobulin G (IgG) are the essential tools in diagnostic kits. The whole-cell is still considered to be used for animal‘s immunization to produce IgG. The goal of this study was to determine dominant protein on the Shiga toxin-producing Escherichia coli (STEC) O157:H7-induced typical antibody response also determined by MALDI-TOF/TOF mass spectrometry. The heating method was conducted at 71 °C and 83 °C for antigen preparation. The proteins were characterized using SDS-PAGE and evaluated by MALDI-TOF/TOF mass spectrometry. The present study reported that the dominant polyclonal antibody inducing protein was ompC E. coli. The evaluation of mass spectra by MALDI-TOF/TOF might help to identify the dominant polyclonal antibody inducing protein in future preparation of diagnostic kits.
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