Oil feeding during the oxytetracycline biosynthesis

The ability of utilization of vegetal oils belongs to common properties of Streptomycetes. During the cultivation of Streptomyces rimosus and biosynthesis of oxytetracycline in 15 m3 fermenters, a fixed regime of soya bean oil feeds as antifoam agent and partial carbon source according to subjectively designed mechanism was investigated and further also feeding regime after exhausting starch as main carbon source and finally his dosing with ammonia sulphate in accordance with physiological activity of the culture. In the second case with semicontinuous supplements of soya bean oil it was possible to prevent premature autolysis of mycelia and to extend the biosynthetic process with keeping the oxytetracycline production rate. The third approach, the most progressive one, represents besides the automatisation of control loop the necessity of assurance continuous pH adjustment for elevation of the process production rate.