阿尔及利亚刺柏(Juniperus oxycedrus)叶、茎挥发油的表征及抗氧化活性

Menad Soumia, Djilali Achour, Moussa Brada
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摘要

在过去的几十年里,精油的使用显著增加,影响着人类日常生活的各个方面(化妆品、制药、农药和食品工业)。本文研究了加氢蒸馏法和水蒸气蒸馏法提取的阿尔及利亚刺柏精油的化学成分和抗氧化活性。比较研究可以突出各种参数对精油收率的影响(干燥,提取时间)。确定了40个成分。气相色谱-质谱分析表明,加氢蒸馏法提取效果较好,主要化学成分为α-蒎烯(37.163%)、沙宾烯(12.829%)和柠檬烯(5.795%)。提取方法表明,对精油的成分有显著影响。刺柏精油具有较强的抗氧化活性。通过DPPH和β-胡萝卜素漂白试验,加氢蒸馏法得到的油的活性分别高于蒸汽夹带法(IC50 = 120 mg ml - 1和IC50 = 160 mg ml - 1)。因此,木犀草精油可以作为抗氧化剂使用。
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Characterisation and Antioxidant Activity of Essential Oils of Leaves and Stems of Juniperus oxycedrus from Mostaganem (Algeria)
In the last decades, the use of essential oils have been increased significantly to affect various aspects of human daily life (cosmetics, pharmacy,pesticide and food industry). The present work aims to study the chemical composition and antioxidant activity of essential oils of Juniperus oxycedrus local (Mostaganem Algeria) extracted by hydrodistillation and steam distillation. The comparative study has permitted to highlight the influence of various parameters on the yield of essential oils (drying, extraction time). Forty components were identified. The characterisation by (GC-MS) showed that the extraction by hydrodistillation gave a better result which was characterised by the presence of α-pinene (37.163 %), sabinene (12.829 %), and limonene (5.795 %) as main chemical constituents. The method of extraction showed that there was a significant influence on the composition of the essential oil. The essential oil of Juniperus oxycedrus showed a strong antioxidant activity. The activity of the oils obtained by hydrodistillation was higher than that obtained by steam entrainment (IC50 = 120 mg ml−1 and IC50 = 160 mg ml−1) with DPPH and β-carotene bleaching tests, respectively. Essential oils of J. oxycedrus could therefore represent good component to be used as an antioxidant agent.
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