原子力声显微镜成像单个肺癌细胞最佳固化条件的确定

2018 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO) Pub Date : 2018-08-01 DOI:10.1109/3M-NANO.2018.8552210

Xuan Wang, Yujing Zhao, Wenxiao Zhang, Ying Wang, Liguo Tian, Xinyue Wang, Zhengxun Song, Zuobin Wang, Dayou Li

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引用次数: 1

摘要

本文提出了原子力声显微镜(AFAM)对单个肺癌细胞成像最佳固化条件的确定方法。用AFAM观察不同浓度的多聚甲醛和甲醇处理细胞的细胞形态、高度和表面粗糙度以及持续时间。实验结果表明，4%多聚甲醛固化30min ~ 60min后的A549细胞与活细胞形态接近。4%多聚甲醛固化10min有利于细胞获得亚表面结构。

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Determination of Optimal Curing Conditions for Imaging Single Lung Cancer Cells by Atomic Force Acoustic Microscope

This paper presents a method for the determination of optimal curing conditions for the imaging of single lung cancer cells by atomic force acoustic microscope (AFAM). The cellular morphology, height and surface roughness of the cells treated with different concentrations of paraformaldehyde and methanol and durations were observed using an AFAM. The experimental results showed that the A549 cells solidified with 4% paraformaldehyde for the period from 30min to 60min were close to the profiles of living cells. The cells solidified with 4% paraformaldehyde for 10min were beneficial to obtain subsurface structures.

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2018 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO)

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