Titer Hemaglutinasi dan Kematian Embrio pada Telur Spesific Antibody Negative (SAN) dengan Usia yang Berbeda Saat Inokulasi Virus Avian Influenza

. Avian influenza (AI) not only infects various species of aves but also can be transmitted to humans. This study was aim to examine hemagglutination titers and embryonic death in Specific Antibody Negative (SAN) eggs with different ages at the time of Avian Influenza (AI) virus inoculation. The study used a completely randomized design with four treatment groups, namely SAN eggs 7 days old, 9 days, 11 days, and 13 days. The treatment was repeated 6 times so the total sample of SAN eggs used was 24 eggs. The results showed that the age of SAN eggs had a significant effect on the hemagglutination titer and the time of death of chicken embryos. Average hemagglutination titer SAN eggs age 9; 11, and 13 days respectively were 597.33 ± 85.33 HAU/25 µ l; 1,322.67 ± 339.73 HAU/25µl, and 256 ± 85.86 HAU/25 µ l. There was no hemagglutination titer in SAN eggs aged 7 days post-inoculation AI virus. The time of embryonic death in SAN eggs aged 7 occurred one-day post-inoculation AI virus, while age 9; 11, and 13 days occurred two days post-inoculation AI virus. In conclusion, hemagglutination titers and embryonic mortality differed depending on the age of the SAN eggs, and the best age to be used for AI virus inoculation was 11 days.