从不同临床标本中分离出的罕见菌种的分离与鉴定

R. Faraj, Muhamed N Maarof
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摘要

临床标本中存在着许多不常见、不常见的机会性细菌种类,其中大多数难以常规鉴定,甚至有些在临床标本中记录较少。在疾病坐标上的作用也不比普通细菌少。从2015年10月至2016年11月期间在苏莱曼尼亚市部分医院和卡拉尔总医院就诊的患者中采集了650份样本。首先将样品在不同培养基上培养,根据培养特性、形态特征和生化反应分离鉴定分离菌,并用Vitek 2系统鉴定罕见和罕见分离菌。鉴定和药敏试验在卡拉尔总医院进行。从不同临床样品中分离到286株(44%)细菌,其中125株经Vitek 2自动鉴定,23株(8%)为罕见菌。罕见菌株对24种抗生素的药敏差异显著。四个隔离;鲍曼动杆菌、钙酸动杆菌、路德维希肠杆菌和血芽孢杆菌对所有抗生素均耐药。其中,尿气球菌、弗氏柠檬酸杆菌和中耳炎异源球菌、摩根氏摩根杆菌、少动藻单胞菌、聚集pantoagglomerans、sobrincoccus和Kocuria roseans .2)、人类ochrobacbacterium n.1和kocurstinae)、人类ochrobacbacterium n.2和Kocuria rosestinae .1、stutzeri假单胞菌、荧光假单胞菌和koseri柠檬酸单胞菌、沙门氏菌气单胞菌和黄体微球菌1和黄体微球菌2对抗生素耐药的比例为95.8%;91.6%, 87.5%, 83.3%, 79.1%, 70.8%, 66.6% and62.5%,分别。
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Isolation and identification of some uncommon bacterial species isolated from different clinical sample
There are many opportunistic bacterial species that are uncommon and infrequently exist inclinical specimen, most of them are difficult to routine identification, even some of them arepoorly documented in clinical specimen. also had no less role in the coordinates of the diseasethan common bacterial species. Six hundred and fifty samples were collected from patientsattending to some hospitals in Sulaimanya City and Kalar General Hospital during the periodfrom October 2015 to November 2016.Samples were firstly cultured on different media in order to isolate and identify bacterialisolates according to cultural characteristics, morphological features and biochemical reactions inaddition to Vitek 2 system for identifying uncommon and infrequent isolates. The identificationand susceptibility test were performed in Kalar General Hospital. Isolated 286(44%) bacterialstrains from different clinical samples, 125 of them were identified by Vitek 2 automatedsystem, while 23(8%) of isolates were considered as uncommon bacterial species.The antimicrobial susceptibility of uncommon isolates, showed significant variation againsttwenty four antibiotics. Four isolates; Acinatobacter baumanii, Acinatobacter calcoaceticus,Enterobacter ludwigii and Gemella sanguinis were resistant to all antibiotics. WhereasAerococcus urinae, (Citrobacter freundii and Alloiococcus otitis), (Morganella morganii,Sphingomonas paucimobilis, Pantoea agglomerans, Streptococcus sobrinus and Kocuria rosean.2), ( Ochrobactrum anthropi n.1 and Kocura kristinae), (Ochrobactrum anthropi n.2 andKocuria rosea n.1 ), (Pseudomonas stutzeri, Pseudomonas fluorescens and Citrobacter koseri ),(Aeromonas salmonicida and Micrococcus luteus n.1) and Micrococcus luteus (n.2) resistant tothe antibiotics as these percentages 95.8%, 91.6%, 87.5%, 83.3%, 79.1%, 70.8%, 66.6% and62.5%, respectively.
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