An investigation of cellular immunity in DMBA induced hamster's tongue neoplasia formation. I. Delayed type hypersensitivity reaction in dinitrochlorobenzene of hamsters.:I. Delayed type hypersensitivity reaction in dinitrochlorobenzene of hamsters

Through the use of 119 3-month-old male hamsters the DNCB delayed type hypersensitivity (DTH) reactions to radiation exposure, doses of anticarcinoagents, and steroids on both normal hamsters and DMBA induced hamsters with tongue neoplasia were examined.Skin of the hamsters' hind liras were sensitized with injections of 5% DNCB-Freund's Incomplete Adjuvant suspension 0.1ml below the skin. One week following the sensitization the skin of each hamster's abdomen was tonsured and comparative challeng testw ere conducted by performing one patch test of 0.5% DNCB acetone solution and another of 0.1ml saline solution for purposes of comparison. The tested portions were then examined 24 hours later.On the untreated normal hamster's DNCB-DTH reactions, 94.9% were positive. The DMBA treated group's DNCB-DTH reactions after the prescribed amount of time had elasped, rose from 90% to 94. 7%. From these results it was surmised that positive-DNCB-DTH reactions were recognized in the DMBA sensitized, DNCB tested hamster group and that an antigeneicity common to both DMBA and DNCB existed. This showed the difficulty investigating degrees of cellular immunity through the DTH reactions of DNCB in the DMBA carcinogenic experimental group.For 14 consecutive days Hydrocorcisone sodium succinate 10mg/kg, Predonisolone sodium succinate 6mg/kg, Cyclophosphamide 20mg/kg, and Tegaful 100mg/kg were injectedinto the peritoneal cavities of normal hamsters which recieved sensitization on the seventh day. On the fourteenth day test results proved that the positive DNCB-DTH reactions had not been decreased.However, the decreas on DNCB-DTH reactions was recognized in the portion of the group which recieved radiation exposure (6 MeV, X-Ray 300 rad, single full body exposure) prior to, and following sensitization.