柠檬酸甲酸菌降解多氯酚的研究

S. Bhatt, R. NichithK, M. Arvind
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摘要

多氯酚是一类广泛用于农药、杀菌剂、杀虫剂等杀菌剂生产的有机化合物,杀菌剂工业的废水中含有大量的多氯酚,污染着环境。多氯酚具有高度毒性,因为它们能够破坏生物膜的结构和功能完整性,造成致命的后果。因此,生物修复为减轻环境污染提供了一种有前途的、省油的结果。细菌和真菌具有在其自然环境中以好氧或厌氧方式降解有机化合物的能力。细菌和真菌可以很容易地代谢多氯酚,产生进入克雷布斯循环的中间体。在本研究中,分离的生物被鉴定为柑橘节杆菌(Arthrobacter citreus),并研究了其降解两种重要的多氯酚(三氯酚和五氯酚)的能力。三氯酚是天然存在的,而;五氯酚是人为的。发现细菌分别在5mM和3mM浓度的三氯苯酚和五氯苯酚下生长。通过薄层色谱分析确定了代谢产物β-酮己二酸,从而保证了代谢成分进入柠檬酸循环。
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Biodegradation of Polychlorophenols By Arthobacter Citreus
Polychlorophenols are a group of organic compounds widely used in the production of biocides such as pesticides, fungicides, insecticides, etc., The effluents from the biocide industry, thus possess a large amount of these Polychlorophenols contaminating the environment. The Polychlorophenols are highly toxic due to their ability to disrupt the structural and functional integrity of biological membranes, causing lethal repercussions. Bioremediation thus provides a promising and skimping outcome to relieve environmental pollution. Bacteria and fungi possess the ability to degrade organic compounds in their natural environment, either aerobically or anaerobically. Bacteria and fungi can easily metabolize Polychlorophenols to produce intermediates that enter the Krebs cycle. In the present study, the isolated organism was identified as Arthrobacter citreus, and its ability to degrade two important Polychlorophenols, i.e., trichlorophenol pentachlorophenol, was investigated. Trichlorophenol is naturally occurring, whereas; pentachlorophenol is anthropogenic. The bacteria were found to grow at 5mM and 3mM concentrations of trichlorophenol and pentachlorophenol, respectively. The metabolites were also determined by Thin Layer chromatography analysis, which indicated the metabolite, β-ketoadipate, thus ensuring the entry of the metabolized components to the citric acid cycle.
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