苋科6个分类群对病原菌分离效果及形态特征的比较研究。

Ahmed, K. Gayar
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引用次数: 1

摘要

本文对苋科6个分类群的抗菌作用和大形态性状进行了比较研究。对其氯仿提取物的抑菌效果进行了测定,并描述了其茎、叶、穗、花和果实的宏观形态特征。采用圆盘扩散试验,肺炎克雷伯菌和巴氏葡萄球菌分别对37%和64%的测试抗生素显示多重耐药。对所选6个分类群进行了短杆菌、巴氏葡萄球菌和肺炎克雷伯菌的抗菌试验。肺炎克雷伯菌对各提取物均敏感。在较低剂量(1.5mg/孔)下,绿苋菜和野田葵提取物对巴氏葡萄球菌的生长有抑制作用。当浓度为3mg/孔时,其对其他提取物的抑制作用开始减弱,而当浓度为6mg /孔时,其抑制作用达到最大。对亚麻短杆菌生长的抑制作用最低的是绿苋菜、水芹、水芹和杂花苋菜,均为1.5 mg/孔。然而,苋菜和绿箭虫在3mg/孔时的抑制作用较低,在6mg /孔时的抑制作用最高。杂交种与绿纹蚜均为近缘种,javanica Aerva与绿纹蚜为远缘种。构建相似指数(SI)和聚类分析,并基于34个性状(22个形态性状和12个抑菌试验)生成物候图。
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Comparative studies between six taxa of Amaranthaceae based on their effects on some pathogenic bacterial isolates and morphological characters.
The present study illustrated the comparative studies among six taxa of Amaranthaceae based on antimicrobial effects and macromorphological traits. The antimicrobial effects data concluded by of their chloroform extracts and the macromorphological characters described were stem, lamina features, spike, flower and fruits. Using disk diffusion assay, Klebsiella pneumoniae and Staphylococcus pasteuri demonstrated a multiple resistance against 37% and 64% of the tested antibiotics, respectively. The selected six taxa were tested as antimicrobial agents against Brevibacterium lines, Staphylococcus pasteuri and Klebsiella pneumonia. Klebsiella pneumoniae was sensitive to all extracts. The growth of Staphylococcus pasteuri was inhibited at a lower dose (1.5mg/well) using Amaranthus viridis and Digera muricata extracts. It had begun inhibition at 3mg/well against the others extracts and reached to the maximum inhibition at 6 mg/well using Amaranthus viridis.The lowest growth of Brevibacterium linens inhibition was at 1.5 mg/well using Amaranthus viridis, Digera muricata, Aerva javanica, and Amaranthus hybridus. However, the lower inhibition using both of Amaranthus graecizans and Aerva lanata was at 3mg/well and gave the highest inhibition with Digera muricata at 6 mg/well. Both A. hybridus and A. viridis were recorded as closely species while Aerva javanica and A. viridis were distantly related species. Similarity index (SI) and cluster analysis were constructed and the phenogram was generated among them based on 34 characters (22 morphological characters and 12 antimicrobal assay).
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