{"title":"动物细胞繁殖中的分子事件","authors":"Kazuto Kajiwara, Gerald C. Mueller","doi":"10.1016/0926-6550(64)90078-7","DOIUrl":null,"url":null,"abstract":"<div><p>Synchronized cultures of HeLa cells were caused during a single cycle of DNA synthesis to replicate given fractions of their DNA with 5-bromodeoxyuridine (BdeU) instead of thymidine. It was observed that the introduction of 5-bromodeoxyuridine into the early replicating DNA complexes resulted in a striking decline in the cloning efficiency whereas the introduction of 5-bromodeoxyuridine into the late replicating DNA was without effect. The data suggest that the early replicating DNA of the HeLa nucleus plays an important role in survival.</p></div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 3","pages":"Pages 486-493"},"PeriodicalIF":0.0000,"publicationDate":"1964-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90078-7","citationCount":"73","resultStr":"{\"title\":\"Molecular events in the reproduction of animal cells\",\"authors\":\"Kazuto Kajiwara, Gerald C. Mueller\",\"doi\":\"10.1016/0926-6550(64)90078-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Synchronized cultures of HeLa cells were caused during a single cycle of DNA synthesis to replicate given fractions of their DNA with 5-bromodeoxyuridine (BdeU) instead of thymidine. It was observed that the introduction of 5-bromodeoxyuridine into the early replicating DNA complexes resulted in a striking decline in the cloning efficiency whereas the introduction of 5-bromodeoxyuridine into the late replicating DNA was without effect. The data suggest that the early replicating DNA of the HeLa nucleus plays an important role in survival.</p></div>\",\"PeriodicalId\":100173,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects\",\"volume\":\"91 3\",\"pages\":\"Pages 486-493\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1964-11-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0926-6550(64)90078-7\",\"citationCount\":\"73\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0926655064900787\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926655064900787","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Molecular events in the reproduction of animal cells
Synchronized cultures of HeLa cells were caused during a single cycle of DNA synthesis to replicate given fractions of their DNA with 5-bromodeoxyuridine (BdeU) instead of thymidine. It was observed that the introduction of 5-bromodeoxyuridine into the early replicating DNA complexes resulted in a striking decline in the cloning efficiency whereas the introduction of 5-bromodeoxyuridine into the late replicating DNA was without effect. The data suggest that the early replicating DNA of the HeLa nucleus plays an important role in survival.
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