Storage Duration Effect of Kelor Leaf (Moringa oleifera) Extracts with Methanol against Growth of Streptococcus agalactiae and Escherichia coli Caused Mastitis in Dairy Cattle

The research aimed to determine the storage duration effectiveness of Moringaoleiferaleaf extract to inhibit the growth of Streptococcus agalactiaeand Escherichia coli causemastitis on dairy cows. The materials were Streptococcus agalactiae and Escherichia coli from Bacteriology Laboratory Agriculture Faculty, Brawijaya University counted as 108 CFU/ml and Moringa oleifera leaf. This research method was an experiment Completely Randomized Design 5 treatments and 5 replications. Storage duration treatment was P0 (control), P1 (2nd day), P2 (4th day), P3 (6th day), P4 (8th day) on the same concentrations 70%. The variable measured was the diameter of the inhibition zone. The data analyzed using ANOVA followed by the Duncan test. The results showed that Moringa oleifera leaf extract had difference highly significant capability to inhibit the growth of Streptococcus agalactiae and Escherichia coli (P < 0.01). The Capability of Moringa leaf extract to maintain bacterial growth inhibition until day 2 for Streptococcus agalactiae and Escherichia coli. Maximum storage time until day 2 to maintain the effectiveness of Moringa leaf extract in inhibiting the growth of Streptococcus agalactiae and Escherichia coli.