黄瓜枯萎病生物防治剂的评价

Deepika Sharma, A. Shukla, Meenu Gupta
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摘要

该试验于2018-2019年5月至8月的种植季节在印度喜马偕尔邦索兰纳乌尼的Yashwant Singh Parmar园艺和林业大学植物病理学系进行,旨在研究真菌和细菌生物防治剂对黄瓜枯萎病的防治效果。在室内条件下,分别采用双培养法和条纹板法测定了不同抑菌剂对尖孢镰刀菌菌丝生长的抑制效果。在真菌生防剂中,哈茨木霉对尖孢镰刀菌菌丝生长的抑制率最高,为61.08%,其次是绿木霉,抑制率为57.51%,而在细菌生防剂中,对荧光假单胞菌的抑制率最高,为45.69%。在种植季节,在田间条件下用有效的真菌和细菌生物制剂对种子和土壤进行处理。哈兹木霉种子处理和土壤施用对黄瓜枯萎病的防治效果为48.25%,其次是绿木霉,防治效果为42%。
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Evaluation of Bio-Control Agents against Fusarium Wilt of Cucumber
The experiment was carried out in Department of Plant Pathology, Dr Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh, India during cropping season of May to August in year 2018–2019 to study the efficacy of fungal and bacterial bio-control agents in managing the Fusarium wilt of cucumber. Different fungal and bacterial bio-control agents were thus tested under laboratory conditions for their efficacy in inhibiting the mycelial growth of Fusarium oxysporum by using dual culture technique and streak plate method, respectively. Among fungal bio-control agents, Trichoderma harzianum was found most effective with 61.08% inhibition of mycelial growth of F. oxysporum followed by Trichoderma virens with 57.51% mycelial inhibition whereas, among bacterial bio-control agents, maximum mycelial inhibition of 45.69% was observed in Pseudomonas fluorescens. During the cropping season, seed, and soil treatment with effective fungal and bacterial bioagents was carried out under field conditions. Seed treatment and soil application of Trichoderma harzianum was found effective with disease control of 48.25% followed by Trichoderma virens which is proved to be efficacious with 42% disease control against Fusarium wilt of cucumber.
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