精浆生物光谱法测定男性不育症精液质量的实验研究

International journal of advancements in computing technology Pub Date : 2016-04-14 DOI:10.4172/0976-4860.1000E007

A. Heidari

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引用次数: 161

摘要

探讨精液中镁(Mg)、磷(P)、钾(K)、钙(Ca)、铬(Cr)、锰(Mn)、铁(Fe)、铜(Cu)、锌(Zn)、硒(Se)、钼(Mo)和碘(I)浓度与精液参数的相关性[1 - 15]。500名可育男性和1000名不育男性提供标准化精液标本。采用火焰原子吸收光谱(FAAS)、衰减全反射傅里叶变换红外光谱(ATR-FTIR)、能量色散X射线光谱(EDX)、紫外可见光谱(UV-Vis)和傅里叶拉曼光谱(FT-Raman)测定了镁(Mg)、磷(P)、钾(K)、钙(Ca)、铬(Cr)、锰(Mn)、铁(Fe)、铜(Cu)、锌(Zn)、硒(Se)、钼(Mo)和碘(I)的浓度。精液分析按照SigmaAldrich公司的指导方针进行。精液中镁(Mg)、磷(P)、钾(K)、钙(Ca)、铬(Cr)、锰(Mn)、铁(Fe)、铜(Cu)、锌(Zn)、硒(Se)、钼(Mo)和碘(I)的浓度呈极显著正相关(P <0.001)。镁(Mg)、磷(P)、钾(K)、钙(Ca)、铬(Cr)、锰(Mn)、铁(Fe)、铜(Cu)、锌(Zn)、硒(Se)、钼(Mo)和碘(I)浓度与活力和运动性呈显著正相关(P <0.0001)。另一方面，镁(Mg)、磷(P)、钾(K)、钙(Ca)、铬(Cr)、锰(Mn)、铁(Fe)、铜(Cu)、锌(Zn)、硒(Se)、钼(Mo)和碘(I)浓度与形态异常呈负相关(图1-3)。因此，目前的数据表明，镁(Mg)、磷(P)、钾(K)、钙(Ca)、铬(Cr)、锰(Mn)、铁(Fe)、铜(Cu)、锌(Zn)、硒(Se)、钼(Mo)和碘(I)浓度可以作为精液质量测定的有用因素。

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An Experimental Biospectroscopic Study on Seminal Plasma in Determination of Semen Quality for Evaluation of Male Infertility

To investigate the correlations among seminal plasma Magnesium (Mg), Phosphorus (P), Potassium (K), Calcium (Ca), Chromium (Cr), Manganese (Mn), Iron (Fe), Copper (Cu), Zinc (Zn), Selenium (Se), Molybdenum (Mo) and Iodine (I) concentrations and semen parameters [1– 15]. 500 fertile and 1000 infertile males provided a standardized semen specimen. Total Magnesium (Mg), Phosphorus (P), Potassium (K), Calcium (Ca), Chromium (Cr), Manganese (Mn), Iron (Fe), Copper (Cu), Zinc (Zn), Selenium (Se), Molybdenum (Mo) and Iodine (I) concentrations were determined by Flame Atomic Absorption Spectroscopy (FAAS), Attenuated Total Reflection Fourier Transform Infrared Spectroscopy (ATR–FTIR), Energy–Dispersive X– Ray Spectroscopy (EDX), UV–Vis spectroscopy and FT–Raman spectroscopy. Semen analysis was performed according to the SigmaAldrich Corporation guidelines. The strong and positive correlation was found among seminal plasma Magnesium (Mg), Phosphorus (P), Potassium (K), Calcium (Ca), Chromium (Cr), Manganese (Mn), Iron (Fe), Copper (Cu), Zinc (Zn), Selenium (Se), Molybdenum (Mo) and Iodine (I) concentrations (p<0.001). Also, positive correlation was found among Magnesium (Mg), Phosphorus (P), Potassium (K), Calcium (Ca), Chromium (Cr), Manganese (Mn), Iron (Fe), Copper (Cu), Zinc (Zn), Selenium (Se), Molybdenum (Mo) and Iodine (I) concentrations and vitality and motility (p<0.0001). On the other hand, there is negative correlation among Magnesium (Mg), Phosphorus (P), Potassium (K), Calcium (Ca), Chromium (Cr), Manganese (Mn), Iron (Fe), Copper (Cu), Zinc (Zn), Selenium (Se), Molybdenum (Mo) and Iodine (I) concentrations and abnormal morphology (Figures 1–3). Therefore, the present data indicated that Magnesium (Mg), Phosphorus (P), Potassium (K), Calcium (Ca), Chromium (Cr), Manganese (Mn), Iron (Fe), Copper (Cu), Zinc (Zn), Selenium (Se), Molybdenum (Mo) and Iodine (I) concentrations could be useful factor in determination of semen quality.

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International journal of advancements in computing technology

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