气相色谱法测定植物提取物成分:姜黄提取物对利什曼原虫的抑制作用和生姜抗利什曼原虫的作用

Hedieh Zadeh-Abbasi Zarandi, L. Shirani-Bidabadi, J. Zolala, Abass Aghaei-Afshar, Ali Faghihi zarandi, Ehsan Salarkia, Mostafa Eghbalian
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引用次数: 0

摘要

姜黄提取物和姜黄精油对多种细菌、寄生虫和病原真菌具有抑制作用。研究生姜和姜黄提取物对promastigotes利什曼原虫的抑制作用,并采用气相色谱-质谱联用技术对植物提取物进行分析。将两种植物的水醇提取物用70%的乙醇稀释成三种不同的浓度;12.5, 100和500 mg mL-1。利什曼原虫显著菌株在人工培养基中繁殖,以达到足够的寄生虫。浸提液浸提时间和浸提液浓度对促毛利什曼原虫存活率有显著影响(P < 0.05)。重复测量模式显示不同时间点与提取物处理之间的相互作用效应。经统计学分析,不同浓度及提取液样品间差异有统计学意义(P < 0.05)。GC-MS结果表明,生姜水酒精提取物在3个时间点(24、48和72 h)处理的promastigotes利什曼原虫的存活率低于葡聚糖和姜黄提取物。姜黄提取物处理的promastigotes的存活率与葡聚糖酶处理的存活率相似,但低于500 mg mL-1浓度的姜和姜黄联合提取物处理的存活率。
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Gas chromatography analysis of plant extracts to examine ingredients: Turmeric extracts on Leishmania Promastigotes and anti-Leishmania effect of Ginger
Turmeric extract and aroma oil of Curcuma longa exhibit inhibition properties against various bacteria, parasites, and pathogenic fungi. We investigated the effects of Ginger (Zingiber officinale) and Turmeric extract on Leishmania promastigotes and used gas chromatography-mass spectrometry (GC-MS) for analyzing plant extracts. The hydroalcoholic extractions obtained from the two plants were diluted in 70% ethanol to three different concentrations; 12.5, 100, and 500 mg mL-1. The Leishmania significant strains were propagated in an artificial medium to reach sufficient parasites. The survival percentage of Leishmania promastigotes was affected significantly by the time and concentration of the extracts (P < 0.05). The repeated measures pattern showed an interaction effect between various time points and treatment with the extracts. Statistics analysis showed a significant difference between different concentrations and extract samples (P < 0.05). GC-MS showed that the survival rate of Leishmania promastigotes treated with hydroalcoholic extract of Ginger at 3-time points (24, 48, and 72 hours) was lower than Glucantime and Turmeric extract. The survival rate of promastigotes treated with Turmeric extract was similar to those treated with Glucantime but lower than those treated with a combined extract of Ginger and Turmeric at a concentration of 500 mg mL-1. 
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