Optimization of the cupric oxide nanoparticles synthesis by novel spondias mombin peel extract exhibited excellent peroxidase activity

This study describes a straightforward protocol for synthesizing cupric oxide nanoparticles from the peel extract of Spondias mombin. The CuO NPs were produced in a basic H2O solution as cubic-shaped, homogenous nanoparticles with excellent peroxidase activity without taking any special precautions. The influence of the concentration of the peel extract, temperature as well as the synthesis medium on CuO NPs production has been investigated extensively. The typical UV-visible absorption maxima observed at wavelengths of 279 and 383 nm observed in spectrophotometry confirmed the formation of CuO NPs. Moreover, the homogeneity, shape (cubic), and size (approximately averaged ⁓31nm (29nm⁓38nm)) of the synthesized CuO nanoparticles under optimum conditions were confirmed through the SEM technique. Besides, its elemental compositions, capping agent, and crystallinity were investigated through    EDS, FT-IR and XRD. Finally, in the presence of hydrogen peroxide (H2O2), the as-prepared CuO NPs demonstrated excellent peroxidase activity against 3,3',5,5'-tetramethylbenzidine (TMB). J. Bangladesh Acad. Sci. 46(2); 135-145: December 2022