{"title":"低温和休眠解除对葱内芽呼吸速率、糖、酚类物质和过氧化物酶活性变化的影响","authors":"N. Benkeblia","doi":"10.1080/09064710310003358","DOIUrl":null,"url":null,"abstract":"Respiration rate (CO 2 production), soluble sugars, total phenolics and peroxidase activity (POD) were measured in inner bud tissues during the break of dormancy of onion bulbs treated for one week at 0°C and stored in the dark at 20°C. Results showed that after eight weeks, the RR C O 2 of sprouted control onions was 52% higher than that of unsprouted control onions. High concentrations of soluble sugars (glucose, fructose and sucrose) were observed for cold treated bulbs (from 9 to 20 mg g - 1 FW) after two weeks following cooling, but in control bulbs high concentrations were only observed after eight weeks. In cold-treated onions, a slight increase in phenolics (from 0.17 to 0.2 mg g - 1 FW) was observed after one week, and then there was a decrease to 0.12 mg g - 1 FW after five weeks. In inner buds of control bulbs, an increase from 0.17 to 0.2 mg g - 1 FW was observed during the first five weeks and then a decrease to 0.15 mg occurred during the three last weeks when the bulbs began to sprout. Peroxidase activity followed the same pattern as phenolics, with a decrease (38%) in cold-treated bulb after four weeks. In control samples, a decrease of 25% was noted after eight weeks. This decrease in POD activity coincided with the decrease in phenolics and the onset of sprouting. With cold treatment, total sprouting was observed after eight weeks. In comparison, only 20% of the control bulbs started sprouting after the same period.","PeriodicalId":40817,"journal":{"name":"Acta Agriculturae Scandinavica Section B-Soil and Plant Science","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"8","resultStr":"{\"title\":\"Low Temperature and Breaking of Dormancy Effects on Respiration Rate, Sugars, Phenolics and Peroxidase Activity Changes in Inner Buds of Onion Allium cepa L.\",\"authors\":\"N. Benkeblia\",\"doi\":\"10.1080/09064710310003358\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Respiration rate (CO 2 production), soluble sugars, total phenolics and peroxidase activity (POD) were measured in inner bud tissues during the break of dormancy of onion bulbs treated for one week at 0°C and stored in the dark at 20°C. Results showed that after eight weeks, the RR C O 2 of sprouted control onions was 52% higher than that of unsprouted control onions. High concentrations of soluble sugars (glucose, fructose and sucrose) were observed for cold treated bulbs (from 9 to 20 mg g - 1 FW) after two weeks following cooling, but in control bulbs high concentrations were only observed after eight weeks. In cold-treated onions, a slight increase in phenolics (from 0.17 to 0.2 mg g - 1 FW) was observed after one week, and then there was a decrease to 0.12 mg g - 1 FW after five weeks. In inner buds of control bulbs, an increase from 0.17 to 0.2 mg g - 1 FW was observed during the first five weeks and then a decrease to 0.15 mg occurred during the three last weeks when the bulbs began to sprout. Peroxidase activity followed the same pattern as phenolics, with a decrease (38%) in cold-treated bulb after four weeks. In control samples, a decrease of 25% was noted after eight weeks. This decrease in POD activity coincided with the decrease in phenolics and the onset of sprouting. With cold treatment, total sprouting was observed after eight weeks. In comparison, only 20% of the control bulbs started sprouting after the same period.\",\"PeriodicalId\":40817,\"journal\":{\"name\":\"Acta Agriculturae Scandinavica Section B-Soil and Plant Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2003-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"8\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Agriculturae Scandinavica Section B-Soil and Plant Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1080/09064710310003358\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Agriculturae Scandinavica Section B-Soil and Plant Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1080/09064710310003358","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 8
摘要
在0°C条件下处理1周,20°C暗室保存,测定了休眠结束后洋葱内芽组织的呼吸速率(CO 2产量)、可溶性糖、总酚类物质和过氧化物酶活性(POD)。结果表明,8周后,发芽对照洋葱的RR - co2含量比未发芽对照洋葱高52%。在冷却两周后,冷处理球茎(从9到20 mg g - 1 FW)中观察到高浓度的可溶性糖(葡萄糖、果糖和蔗糖),但在对照球茎中,只有在8周后才观察到高浓度。冷处理洋葱的酚类物质在一周后略有增加(从0.17 mg g - 1 FW增加到0.2 mg g - 1 FW),然后在五周后下降到0.12 mg g - 1 FW。在对照鳞茎内芽中,前5周从0.17 mg g - 1 FW增加到0.2 mg g - 1 FW,然后在鳞茎开始发芽的最后3周下降到0.15 mg。过氧化物酶活性与酚类物质具有相同的模式,4周后冷处理球茎的过氧化物酶活性下降(38%)。在对照样本中,8周后下降了25%。POD活性的下降与酚类物质的减少和发芽的开始一致。冷处理8周后观察总发芽。相比之下,只有20%的对照球茎在同一时期开始发芽。
Low Temperature and Breaking of Dormancy Effects on Respiration Rate, Sugars, Phenolics and Peroxidase Activity Changes in Inner Buds of Onion Allium cepa L.
Respiration rate (CO 2 production), soluble sugars, total phenolics and peroxidase activity (POD) were measured in inner bud tissues during the break of dormancy of onion bulbs treated for one week at 0°C and stored in the dark at 20°C. Results showed that after eight weeks, the RR C O 2 of sprouted control onions was 52% higher than that of unsprouted control onions. High concentrations of soluble sugars (glucose, fructose and sucrose) were observed for cold treated bulbs (from 9 to 20 mg g - 1 FW) after two weeks following cooling, but in control bulbs high concentrations were only observed after eight weeks. In cold-treated onions, a slight increase in phenolics (from 0.17 to 0.2 mg g - 1 FW) was observed after one week, and then there was a decrease to 0.12 mg g - 1 FW after five weeks. In inner buds of control bulbs, an increase from 0.17 to 0.2 mg g - 1 FW was observed during the first five weeks and then a decrease to 0.15 mg occurred during the three last weeks when the bulbs began to sprout. Peroxidase activity followed the same pattern as phenolics, with a decrease (38%) in cold-treated bulb after four weeks. In control samples, a decrease of 25% was noted after eight weeks. This decrease in POD activity coincided with the decrease in phenolics and the onset of sprouting. With cold treatment, total sprouting was observed after eight weeks. In comparison, only 20% of the control bulbs started sprouting after the same period.
期刊介绍:
Acta Agriculturæ Scandinavica Section B publishes original research in applied soil and plant science with special attention given to to crop production in agri- and horticultural systems. We welcome manuscripts dealing with:
Climate smart and sustainable crop production systems
Water and nutrient efficiency
Soil conservation and productivity
Precise agriculture systems
Applications of bio- and nanotechnology
Digitalisation and robotics
Soil-plant interactions
Acta Agriculturæ Scandinavica, Section B – Soil & Plant Science forms part of a series of titles published on behalf of the Nordic Association of Agricultural Science (NJF). The series also includes Section A - Animal Science .