巨型芽孢杆菌降解偶氮染料及其植物毒性研究

Lekha R, Dr.Maleeka Begum, Dr.R. Ragunathan
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引用次数: 9

摘要

各种纺织工业释放的偶氮染料对环境造成了严重的问题。生物降解染料的方法优于化学和物理方法。在最佳条件下,研究了巨芽孢杆菌(MTCC 8371)对亚甲基蓝、甲基红、刚果红和橙-G四种偶氮染料的降解潜力。采用Kirk’s培养基进行降解研究,发现在中性pH、40℃条件下,培养第5天,微生物在Kirk’s培养基中对染料的降解效果最好。用紫外-可见分光光度计对其降解进行了评价。并用FTIR研究了样品中降解的化合物。采用薄层色谱法测定降解染料的rf值。通过SDS-PAGE鉴定了漆酶的分子量,并通过漆酶测定进一步证实了漆酶的降解。GC -MS分析进一步证实了降解。
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Biodegradation of Azo Dyes Using Bacillus Megaterium and Its Phytotoxicity Study
The release of azo dyes from various textile industries contributes a serious problem to the environment. The biological method of degrading these dyes is preferred over chemical and physical methods. In the present study, the potentiality of using Bacillus megaterium (MTCC 8371) for degrading four different azo dyes, namely methylene blue, methyl red, congo red, and orange –G, under optimum conditions were studied. Kirk’s media was used for the degradation study and it was found out that the organism degraded the dye best in Kirk’s media on the fifth day of incubation under neutral pH at 40oC. The degradation was estimated by using UV-VIS spectrophotometer. FTIR study was also carried out to find out the degraded compounds in the samples. TLC was performed to find out the Rƒ value of the degraded dyes. The molecular weight of laccase enzyme was identified by SDS-PAGE and the degradation was further conformed by laccase assay. GC –MS analysis was also performed for the further confirmation of degradation.
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