Y. Cho, S. Lee, B. Le Pioufle, N. Takama, S. Takeuchi, T. Fujii, B.J. Kim
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High-Throughput Single-Cell Electroporation Microchip with Three Dimensional Si Microelectrodes for Gene Transfection
We present details on fabrication of electroporation microchip, practical experiments of single-cell electroporation with our fabricated microchip. Also, the continuous electroporation for the continuous flow of cells is used for high-throughput electroporation. The delivery efficiency and cell viability tests are provided and the successful GFP transfection into cells is also evaluated with a fluorescent microscope after electroporation.
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