两种药用植物海蛾提取物的抗氧化活性及生物学研究

M. Wahba, Hanan S. Gaballa
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引用次数: 2

摘要

利用化学杀虫剂对沿海夜蛾(Spodoptera littoralis, Boisd)虫害进行控制管理。然而,在现代害虫管理策略中,没有这些化学物质不良副作用的环境友好型方法变得非常重要。包括植物乙醇提取物和精油在内的天然产品是防治害虫的一些替代方法。提取精油,用GC/MS谱联用仪鉴定其化学成分。因此,本研究采用2,2 -二苯基-1-苦酰肼(DPPH)法对鼠尾草和百里香等植物提取物的抗氧化活性进行了评价。结果表明,与合成标准丁基羟基甲苯(BHT)相比,25、50、100和200µg/ml浓度的两种植物精油和乙醇提取物在30 min后清除自由基的效率更高。以100、200µg/mL乙醇提取物和精油提取物为对照品,研究了鼠尾草和百里香提取物对棉花叶虫和滨夜蛾的毒力、幼虫的累积死亡率和对4龄滨夜蛾的潜在毒力。结果表明,两种植物的乙醇提取物在不同生长阶段均比精油更有效。结果表明,各处理和对照在某些生物学方面存在极显著差异。
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Antioxidant Activity and Biological Studies of Two Medicinal Plant Extracts on Spodoptera littoralis (Boisd)
Control management of Spodoptera littoralis (Boisd) infestation has been achieved by using chemical insecticides. However, the environment-friendly methods without unwanted side effects of these chemicals are becoming very important in modern pest management strategies. Natural products including plant ethanolic extracts and essential oils are some of the alternative approaches in pest control. The essential oils were extracted and their chemical composition was identified using a GC/MS spectrometer. Therefore, the present study was designed to evaluate the antioxidant activity of various extracts of sage and thyme plants by 2, 2-diphenyl-1-picrylhydrazil (DPPH) method. The obtained results revealed that concentrations at 25, 50, 100 and 200µg/ml of essential oils and ethanolic extract from both plants were more efficient in scavenging free radicals after 30 min when compared with butyl hydroxyl toluene(BHT) as synthetic standard. The concentrations 100 and 200µg/mL from ethanolic and essential oils were having higher activity than other extracts and standard .Furthermore, Sage and thyme extracts were investigated for their toxicity against cotton leafworm, S. littoralis in the laboratory, the percentages of cumulative mortality of larvae as well as the latent effects of the 4th instar of S. littoralis four concentrations (25, 50, 100 and 200 µg/mL) were applied. It was found that ethanolic extract of two plants was more efficient than essential oils on different stages of S. littoralis. The results showed that there were highly significant differences between all treatments and control some biological aspects.
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