母种猪在基因-生产力标记上的模型遗传谱

O. Vasilyuk, I. Sheiko, I. Gridyushko
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引用次数: 0

摘要

目前,分子遗传学和生物学的发展允许直接在DNA水平上进行基因组分析和选择(标记依赖选择)。本研究的目的是建立用于猪育种的计划母种猪产量数量性状标记基因的模型遗传谱。这些品种在白俄罗斯共和国如下:白俄罗斯大白,白俄罗斯黑白和约克郡。研究于2002年至2018年期间在扎德涅普罗夫斯基农业股份有限公司、奥尔尚斯基面包生产厂、JSC、扎雷切耶农业股份有限公司、扎帕德尼农业股份有限公司对白俄罗斯大白猪、白俄罗斯黑白猪和白俄罗斯植物型约克郡猪的纯种动物进行。对母种母猪、公猪和育肥猪进行基因检测。以猪耳廓组织样品为起始材料,在分子生物技术和DNA检测实验室(白俄罗斯国家科学院动物育种研究与实践中心)和动物遗传学实验室(白俄罗斯国家科学院遗传与细胞学研究所)分离并优化DNA,采用PCR - RFLP方法进行基因多态性分析。在已建立的多态性研究基础上,建立了猪母种生产力数量性状标记基因的模式遗传谱。在三个母种中,每个标记基因的首选基因型达到的最大水平作为被评估品种(繁殖幼畜)的模型概况。对于已确定具有高水平标记基因多态性和生产力的品种,已开发出超过已实现指标8- 10pp的模型。
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Model genetic profiles of pigs of mother breeds on genes - productivity markers
Currently, development of molecular genetics and biology allows for genomic analysis and selection directly at the DNA level (marker-dependent selection). The aim of the research was to develop model genetic profiles for marker genes of quantitative traits of productivity of pigs of planned maternal breeds used in pig breeding. Such breeds in the Republic of Belarus are as follows: Belarusian large white, Belarusian black and white and Yorkshire. The studies were carried out at the agricultural branch of Zadneprovsky SGC, Orshansky Plant of Bread Products, JSC, Zarechye SGC, Zapadny SGC with populations of purebred animals of Belarusian Large White, Belarusian Black and White and Belarusian plant type of Yorkshire pigs during 2002-2018. Genetic testing was carried out with sows, boars and finishing pigs of maternal breeds. As a starting material, tissue samples from the auricle of pigs were used with DNA isolated and optimized for the analysis of gene polymorphism using PCR - RFLP method in the laboratories of molecular biotechnology and DNA testing (Research and Practical Center of the National Academy of Sciences of Belarus for Animal Breeding) and animal genetics (Institute of Genetics and Cytology of the National Academy of Sciences of Belarus). As a result of studies based on the established polymorphism, model genetic profiles of maternal breeds of pigs were developed for marker genes of quantitative traits of productivity. The maximum achieved level of the preferred genotype for each marker gene among the three maternal breeds served as a model profile for the evaluated breeds (breeding young animals). For breeds with an established high level of marker gene polymorphism and productivity, a model profile has been developed that exceeds the achieved indicator by 8-10 p.p.
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