基于简单序列重复标记的羊草(禾科,小麦科)系统发育关系及多样性

Xinyi Zhang, Ying‐Xia Lei, Ruiwu Yang
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摘要

目的:研究:(1)羊草Ns基因组供体,阐明羊草Xm基因组的起源;(2)评价这些物种之间的系统发育关系。方法:采用CTAB(十六烷基三甲基溴化铵)法从新鲜叶片组织中提取总基因组DNA。利用150对SSR引物筛选产生多态性的DNA条带,以羊草属植物和13种二倍体多年生植物为模板进行进一步分析。在NTSYS-pc程序中,采用带算术平均的非加权对群法(UPGMA)和顺序、分层、凝聚和嵌套聚类(SHAN)例程构建树形图。结果:引物WMC475、WMC11和QWM213在研究中扩增效率较高。基于SSR标记,羊草与近缘二倍体小麦品种间存在显著的多样性和多态性。最大GS系数值在Pse。针叶草和羊草的遗传距离最近,亲缘关系更密切。在进化支I中,羊草种首先形成了一个很好的支撑点,形成了一个小而独特的类群(Ia)。其中总状L.、盐状L.、特殊L.和小三角L.聚集较紧密。结论:黄芪多糖;黄山尼卡和Psa。fragilis (Ns基因组)与羊草属聚在一起,进一步说明含有Ns基因组的羊草属与Psathyrostachys的亲缘关系更为密切。St基因组和J基因组未参与羊草的起源,羊草物种的亲缘关系和遗传距离与地理分布和环境有关。
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Phylogenetic Relationships and Diversity in Leymus (Poaceae, Triticeae) Based on Simple Sequence Repeats Markers
Aims: The objective of the study was to investigate: (1) the Ns genome donor and elucidate the origins of the Xm genome of Leymus; (2) evaluate the phylogenetic relationships among these species. Methodology: The CTAB (cetyltrimethylammonium bromide) procedure was used to extract total genomic DNA from fresh leaf tissue. A total of 150 pairs of SSR primers were tested to screen those produced polymorphic DNA bands to continue further analysis with Elymus species and 13 diploid perennial species as templates. The GS was used to construct a dendrogram via the unweighted pair group method with arithmetic average (UPGMA) and the SHAN (sequential, hierarchical, agglomerative, and nested clustering) routine in the NTSYS-pc program. Results: The primers WMC475, WMC11 and QWM213 showed more expansion efficiency in the research. There were significant diversity and polymorphism between Leymus and related diploid Triticeae species based on SSR makers. The largest GS coefficient values between Pse. stipifolia and Elymus hystrix indicates that the genetic distance is the closest and has a closer genetic relationship. In clade I, the Leymus species formed a very wellsupported into a small distinct group (Ia) first. Specifically, L. racemosus, L. salinus, L. secalinus and L. triticoides were clustered closely. Conclusion: Psathyrostachys juncea, Psa. huanshanica and Psa. fragilis (Ns genome) clustered together into clade I with Leymus species, which further illustrates Leymus that contains Ns genome is more closely related to Psathyrostachys. St genome and J genome did not participate in the origin of Leymus, and the genetic relationship and genetic distance of Leymus species are related to geographical distribution and environment.
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