李果实抗氧化活性的评价皮肤提取物用于天然酸碱指示剂

Elkawnie Pub Date : 2019-06-29 DOI:10.22373/EKW.V5I1.4516
S. Akbar
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引用次数: 3

摘要

用100 mL乙醇浸渍法提取李子皮。该工艺获得5%(50µg/µL)的皮肤提取物。紫外可见光谱结果表明,花青素提取物在酸性介质中的主吸收峰位于λmax = 526 nm处,这是由花青素类化合物黄酮类化合物的电子跃迁引起的。花青素对温度稳定性的影响表现为吸光度随加热时间的延长而降低,而色素的失色程度或失色百分率逐渐增加。颜料在100℃下保持100 min,损失率达58.5%。因此,在较低的温度下,李子果皮中花青素的颜色损失相对稳定。在pH值为1 ~ 3时,李子皮提取物呈红色,在pH值为4 ~ 7时几乎无色,在pH值为12时呈紫色。最后,梅子果皮提取物可作为酸碱指示剂。
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Evaluation of Antioxidant Activity On Plum Fruit (Prunus domestica L.) Skin Extract Applied For Natural Acid-Base Indicator
Plum peel was extracted through the maceration method with 100 mL ethanol. This process obtained 5% (50 µg/µL) skin extract. The UV-Vis spectrum of the extract displayed the major absorption peak at λmax = 526 nm in acid media, this assigned by the electronic transition from flavylium which as characteristic of anthocyanin compounds. The of anthocyanins stability on temperature effect showed the absorbance decreases with the heating time, while the level of pigment color loss, or the percentage of color loss, gradually increases. The pigment was maintained at 100oC for 100 min with the loss rate reaches 58.5%. Therefore, The loss of anthocyanin color in the plum peel is relatively stable at lower temperatures. Then, The plum peels extract shown the red color at pH 1-3, almost colorless at pH 4-7, and violet color at pH 12. Finally, plum peels extract was potentially used as the acid-base indicator.
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