Hamid Shirkhanloo , Mehri Ghazaghi , Mohammad Mehdi Eskandari
{"title":"云点辅助分散离子液体-基于异丙基2-[(异丙氧基碳硫基)二磺胺基]乙烷硫酸盐的人体血液样品中铬形态的液体微萃取","authors":"Hamid Shirkhanloo , Mehri Ghazaghi , Mohammad Mehdi Eskandari","doi":"10.1016/j.ancr.2016.10.002","DOIUrl":null,"url":null,"abstract":"<div><p>An efficient and fast method based on isopropyl 2-[(isopropoxycarbothiolyl)disulfanyl] ethane thioate (IICDET) were used for the speciation and determination of trace amount of Cr(III and VI) in human biological samples by cloud point assisted dispersive ionic liquid –liquid microextraction (CP-DILLME). Cr(VI) has carcinogenic effects, so, speciation of chromium in human body such as blood cells is very important. The cloudy solution was achieved by the mixture of acetone and IL ([C<sub>8</sub>MIM][PF<sub>6</sub>]) in human blood samples containing Cr(III) ions that were already complexed by IICDET at pH 4.5. After reduction Cr(VI) to Cr(III) by ascorbic acid, chromium speciation was obtained based on total chromium determination by electro thermal atomic absorption spectrometry (ET-AAS) and difference between total Cr and Cr(III) content. In addition, Cr speciation in human blood cells was calculated based on IICDET/CP-DILLME and hematocrit blood test (HCT). After optimized conditions, the enrichment factor (EF), Linear range and limit of detection (LOD) was obtained 25.2, 0.02–1.75 μg L<sup>−1</sup> and 5.4 ng L<sup>−1</sup> in human biological samples respectively. The validation of methodology was achieved by certified reference material (CRM) and ICP-MS technique.</p></div>","PeriodicalId":7819,"journal":{"name":"Analytical Chemistry Research","volume":"10 ","pages":"Pages 18-27"},"PeriodicalIF":0.0000,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ancr.2016.10.002","citationCount":"27","resultStr":"{\"title\":\"Cloud point assisted dispersive ionic liquid -liquid microextraction for chromium speciation in human blood samples based on isopropyl 2-[(isopropoxycarbothiolyl)disulfanyl] ethane thioate\",\"authors\":\"Hamid Shirkhanloo , Mehri Ghazaghi , Mohammad Mehdi Eskandari\",\"doi\":\"10.1016/j.ancr.2016.10.002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>An efficient and fast method based on isopropyl 2-[(isopropoxycarbothiolyl)disulfanyl] ethane thioate (IICDET) were used for the speciation and determination of trace amount of Cr(III and VI) in human biological samples by cloud point assisted dispersive ionic liquid –liquid microextraction (CP-DILLME). Cr(VI) has carcinogenic effects, so, speciation of chromium in human body such as blood cells is very important. The cloudy solution was achieved by the mixture of acetone and IL ([C<sub>8</sub>MIM][PF<sub>6</sub>]) in human blood samples containing Cr(III) ions that were already complexed by IICDET at pH 4.5. After reduction Cr(VI) to Cr(III) by ascorbic acid, chromium speciation was obtained based on total chromium determination by electro thermal atomic absorption spectrometry (ET-AAS) and difference between total Cr and Cr(III) content. In addition, Cr speciation in human blood cells was calculated based on IICDET/CP-DILLME and hematocrit blood test (HCT). After optimized conditions, the enrichment factor (EF), Linear range and limit of detection (LOD) was obtained 25.2, 0.02–1.75 μg L<sup>−1</sup> and 5.4 ng L<sup>−1</sup> in human biological samples respectively. The validation of methodology was achieved by certified reference material (CRM) and ICP-MS technique.</p></div>\",\"PeriodicalId\":7819,\"journal\":{\"name\":\"Analytical Chemistry Research\",\"volume\":\"10 \",\"pages\":\"Pages 18-27\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2016-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.ancr.2016.10.002\",\"citationCount\":\"27\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytical Chemistry Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2214181216300180\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Chemistry Research","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214181216300180","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 27
摘要
采用云点辅助分散离子液体-液体微萃取(CP-DILLME),建立了以2-[(异丙氧基碳硫基)二磺胺基]乙烷硫酸酯(IICDET)为基础的高效、快速测定人体生物样品中微量Cr(III和VI)的方法。铬(VI)具有致癌作用,因此,铬在人体内如血细胞中的形态是非常重要的。混浊溶液是由丙酮和IL ([C8MIM][PF6])的混合物在人血液样品中得到的,其中含有已经在pH为4.5的情况下被IICDET络合的Cr(III)离子。用抗坏血酸将Cr(VI)还原为Cr(III)后,根据电热原子吸收光谱法(ET-AAS)测定总铬和总铬与Cr(III)含量的差异得到铬的形态。此外,基于IICDET/CP-DILLME和hematocrit blood test (HCT)计算人血细胞中Cr的形态形成。优化条件后,在人体生物样品中的富集系数(EF)为25.2,线性范围为0.02 ~ 1.75 μg L−1,检出限(LOD)为5.4 ng L−1。采用标准物质(CRM)和ICP-MS技术对方法学进行验证。
Cloud point assisted dispersive ionic liquid -liquid microextraction for chromium speciation in human blood samples based on isopropyl 2-[(isopropoxycarbothiolyl)disulfanyl] ethane thioate
An efficient and fast method based on isopropyl 2-[(isopropoxycarbothiolyl)disulfanyl] ethane thioate (IICDET) were used for the speciation and determination of trace amount of Cr(III and VI) in human biological samples by cloud point assisted dispersive ionic liquid –liquid microextraction (CP-DILLME). Cr(VI) has carcinogenic effects, so, speciation of chromium in human body such as blood cells is very important. The cloudy solution was achieved by the mixture of acetone and IL ([C8MIM][PF6]) in human blood samples containing Cr(III) ions that were already complexed by IICDET at pH 4.5. After reduction Cr(VI) to Cr(III) by ascorbic acid, chromium speciation was obtained based on total chromium determination by electro thermal atomic absorption spectrometry (ET-AAS) and difference between total Cr and Cr(III) content. In addition, Cr speciation in human blood cells was calculated based on IICDET/CP-DILLME and hematocrit blood test (HCT). After optimized conditions, the enrichment factor (EF), Linear range and limit of detection (LOD) was obtained 25.2, 0.02–1.75 μg L−1 and 5.4 ng L−1 in human biological samples respectively. The validation of methodology was achieved by certified reference material (CRM) and ICP-MS technique.