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{"title":"人多肽/组氨酸转运蛋白1 (hPHT1/SLC15A4)功能的评估:在hCMEC/D3血脑屏障细胞系中利用hPHT1 shRNA稳定转染敲低的转运动力学","authors":"G. Knipp, D. Lindley, Stephen M. Carl, Stephanie A. Mowery, W. Roth","doi":"10.15406/ppij.2021.09.00335","DOIUrl":null,"url":null,"abstract":"Abbreviations: ABC, ATP-binding cassette; A, apical; B, basolateral; BLQ, below the limit of quantitation; EGM, endothelial growth medium; GAPDH, glyceraldehyde-3 phosphate dehydrogenase; GI, gastrointestinal; HBSS, Hank’s balanced salt solution; hPepT, human oligopeptide transporter; hPHT, human peptide/histidine transporter; MES, 2-(N-morpholino)ethanesulfonic acid; MDR, multidrug resistance; MRP, multidrug resistance-like protein; qRT-PCR, quantitative reverse transcriptase-polymerase chain reaction; P-gp, P-glycoprotein; POT, proton-dependent oligopeptide transporter; RP L13a, ribosomal protein L13a; SLC, solute carrier transporter","PeriodicalId":19839,"journal":{"name":"Pharmacy & Pharmacology International Journal","volume":"332 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of human peptide/histidine transporter 1 (hPHT1/SLC15A4) function: transport kinetics utilizing a HPHT1 shRNA stably transfected knockdown in the hCMEC/D3 blood-brain barrier cell line\",\"authors\":\"G. Knipp, D. Lindley, Stephen M. Carl, Stephanie A. Mowery, W. Roth\",\"doi\":\"10.15406/ppij.2021.09.00335\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abbreviations: ABC, ATP-binding cassette; A, apical; B, basolateral; BLQ, below the limit of quantitation; EGM, endothelial growth medium; GAPDH, glyceraldehyde-3 phosphate dehydrogenase; GI, gastrointestinal; HBSS, Hank’s balanced salt solution; hPepT, human oligopeptide transporter; hPHT, human peptide/histidine transporter; MES, 2-(N-morpholino)ethanesulfonic acid; MDR, multidrug resistance; MRP, multidrug resistance-like protein; qRT-PCR, quantitative reverse transcriptase-polymerase chain reaction; P-gp, P-glycoprotein; POT, proton-dependent oligopeptide transporter; RP L13a, ribosomal protein L13a; SLC, solute carrier transporter\",\"PeriodicalId\":19839,\"journal\":{\"name\":\"Pharmacy & Pharmacology International Journal\",\"volume\":\"332 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-06-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pharmacy & Pharmacology International Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.15406/ppij.2021.09.00335\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmacy & Pharmacology International Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15406/ppij.2021.09.00335","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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Evaluation of human peptide/histidine transporter 1 (hPHT1/SLC15A4) function: transport kinetics utilizing a HPHT1 shRNA stably transfected knockdown in the hCMEC/D3 blood-brain barrier cell line
Abbreviations: ABC, ATP-binding cassette; A, apical; B, basolateral; BLQ, below the limit of quantitation; EGM, endothelial growth medium; GAPDH, glyceraldehyde-3 phosphate dehydrogenase; GI, gastrointestinal; HBSS, Hank’s balanced salt solution; hPepT, human oligopeptide transporter; hPHT, human peptide/histidine transporter; MES, 2-(N-morpholino)ethanesulfonic acid; MDR, multidrug resistance; MRP, multidrug resistance-like protein; qRT-PCR, quantitative reverse transcriptase-polymerase chain reaction; P-gp, P-glycoprotein; POT, proton-dependent oligopeptide transporter; RP L13a, ribosomal protein L13a; SLC, solute carrier transporter
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