番茄抗蛇皮霉促生内生微生物的筛选与鉴定

S. U, K. G., Sridharan A P
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引用次数: 0

摘要

从番茄植株组织中分离到45种细菌、9种真菌、3种酵母菌和1种放线菌,对它们的促生长特性和对消霉霉的拮抗活性进行了筛选。在双平板和倒置平板试验中,分离的短小芽孢杆菌TEB10对病原菌的抑制率分别为55%和79%,而分离的热带念珠菌TEY1对病原菌的抑制率分别为30%和68%。此外,短小芽孢杆菌TB10还具有铁载体、HCN、纤维素酶和磷酸盐溶解活性。同样,酵母分离物热带假丝酵母TEY1也具有过氧化氢酶、磷酸盐和锌的增溶活性。甾醇生物合成途径的最终产物麦角甾醇在假单胞菌的次级代谢产物中被分析,表明这种发病机制的可能性。
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Screening and Characterization of Plant Growth Promoting Endophytic Microbes of Tomato (Solanum lycopersicum L.) combats Pythium aphanidermatum
About 45 bacteria, 9 fungi, 3 yeast, and 1 actinobacterium were isolated from plant tissues of tomato and screened for their plant growthpromoting traits and antagonistic activity against damping-off fungus Pythium aphanidermatum. The bacterial isolate, Bacillus pumilus TEB10 exhibited pathogen inhibition of about 55 and 79%, while yeast isolate Candida tropicalis TEY1 showed 30 and 68% in the dual plate and inverted plate assay, respectively. In addition, the Bacillus pumilus TB10 showed siderophore, HCN, cellulase, and phosphate solubilization activities. Similarly, yeast isolate Candida tropicalis TEY1 exhibited catalase, phosphate, and zinc solubilization activities. The sterol biosynthesis pathway end product ergosterol was profiled in secondary metabolites of P. aphanidermatum, suggesting the potentiality of this pathogenesis.
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