生物炭吸附热稳定性研究

H. Noritomi, Ryotaro Kai, N. Endo, Satoru Kato, K. Uchiyama
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引用次数: 1

摘要

研究发现,蛋清溶菌酶(HEWL)在生物炭竹炭粉(BCP)上吸附后,其耐热性明显增强。即使在高温下,hhl也能牢固地吸附在BCP上。在BCP的吸附作用下,HEWL的二级结构发生了一定程度的改变。bcp吸附的hel在900℃下孵育30 min后,剩余活性可达20%以上,而游离的hel几乎没有剩余活性。bcp吸附的hel在900℃下的半衰期为13 min,而游离的hel的半衰期为4 min。
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Thermal Stabilization of HEWL by Adsorption on Biochar
We have found that the heat stress tolerance of hen egg white lysozyme (HEWL) is markedly enhanced by the adsorption of HEWL on bamboo charcoal powder (BCP), which is a kind of biochar. HEWL was firmly adsorbed on BCP even at high temperatures. The secondary structure of HEWL was altered to some extent by the adsorption of HEWL on BCP. The remaining activity of BCP-adsorbed HEWL exhibited more than 20% after the incubation for 30 min at 900C although that of free one was hardly observed. Moreover, the half-life of BCP-adsorbed HEWL was 13 min at 900C while that of free one was 4 min.
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