iTRAQ-Based Quantitative Proteomics Analysis Reveals the Invasion Mechanism of Spiroplasma eriocheiris in 3T6 Cells

Spiroplasma eriocheiris is a novel pathogen of freshwater crustaceans and is closely related to S. mirum. They have no cell wall and a helical morphology. They have the ability to infect mammals with an unclear mechanism. In this study, our aim was to investigate the profile of protein expression in 3T6 cells infected with S. eriocheiris. The proteome of 3T6 cells infected by S. eriocheiris was systematically investigated by iTRAQ. We identified and quantified 4915 proteins, 67 differentially proteins were found, including 30 up-regulated proteins and 37 down-regulated proteins. GO term analysis shows that dysregulation of adhesion protein , interferon and cytoskeletal regulation are associated with apoptosis. Adhesion protein Vcam1 and Interferon-induced protein GBP2, Ifit1, TAPBP, CD63 ,Arhgef2 were up-regulated. A key cytoskeletal regulatory protein, ARHGEF17 was down-regulated. KEGG pathway analysis showed the NF-kappa B signaling pathway, the MAPK signaling pathway , the Jak-STAT signaling pathway and NOD-like receptor signaling are closely related to apoptosis in vivo. Analysis of the signaling pathways involved in invasion may provide new insights for understanding the infection mechanisms of S. eriocheiris.