黄麻飞荷叶中水解单宁的组成及含量

T. A. Krol, D. Baleev, V. I. Ossipov
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摘要

介绍。黄柳属桃金娘科,是一种高4-6米的常绿灌木或小树。黄连果实的植物化学成分研究得最为彻底。研究表明,feijoa富含酚酸、可水解缩合单宁、甾体皂苷和类黄酮。已知可水解单宁具有很高的生物活性。目的:研究黄麻叶中可水解单宁的组成及含量。材料和方法。超高性能液相色谱与二极管阵列检测和质谱相结合。结果和讨论。结果表明,黄连叶提取物中存在22种酚类化合物。它们被分类为黄烷-3-醇、类黄酮和水解单宁。有两种化合物无法鉴定。水解单宁的总含量为44.28 mg/g(占总酚含量的90%)。同时,鞣花丹宁是提取物中主要的酚类化合物(40,47 mg/g),而没食子葡萄糖的含量仅为3,81 mg/g。对VILAR大棚引种黄麻叶片中水解单宁的组成和含量进行了研究。叶片主要积累单体鞣花单宁,占总可水解单宁含量的92%。结果表明,黄花蒿叶可作为提取鞣花单宁高含量提取物的原料,并以此为基础开发药物。
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Composition and Content of Hydrolysable Tannins in Feijoa Leaves, Acca sellowiana
Introduction. Acca sellowiana, or feijoa belongs to the Myrtaceae family and it is an evergreen shrub or small tree 4–6 meters high. The phytochemical composition has been most thoroughly studied in the fruits of A. sellowiana. It has been shown that feijoa is rich in phenolic acids, hydrolysable and condensed tannins, steroidal saponins, and flavonoids. Hydrolysable tannins are known to have high biological activity.Aim. To study the composition and content of hydrolysable tannins in the leaves of A. sellowiana.Materials and methods. Ultra-high performance liquid chromatography coupled with diode array detection and mass spectrometry.Results and discussion. The results obtained showed the presence of 22 phenolic compounds in the extract of the A. sellowiana leaves. They have been classified as flavan-3-ols, flavonoids and hydrolysable tannins. Two compounds could not be identified. The total content of hydrolysable tannins was 44,28 mg/g (90 % of the total phenolic content). At the same time, ellagitannins were the main phenolic compounds of the extract (40,47 mg/g), while the amount of galloyl-glucose was only 3,81 mg/g.Conclusions. Composition and content of hydrolysable tannins in the leaves of A. sellowiana introduced in the greenhouse of VILAR have been studied. The leaves accumulate mainly monomeric ellagitannins represented 92 % of the total content of hydrolysable tannins. The data obtained indicate that the leaves of A. sellowiana can be used as a raw material for obtaining extracts with a high content of ellagitannins and developing drugs based on them.
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