蔬菜乙醇提取物对黄萎病菌的体外防治效果研究

Fernando Sernaqué Fernando Sernaqué, Ana Apaza, Luis Solis, W. Auccahuasi
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摘要

本研究评价了葱、葱和生姜的乙醇提取物对玉米枯萎菌菌丝生长的影响,说明该植物病原菌具有侵染和致病的能力。采用琼脂稀释法,提取液和剂量重复5次,分别在3个不同的时间进行测定,采用双因素方差分析实验设计,显著水平为α = 0.05。当剂量为13% (V/ V)时,Allium sativum乙醇提取物对真菌菌丝生长的抑制率为100%;当剂量为11% (V/ V)时,第5天Allium sativum乙醇提取物对真菌菌丝生长的平均抑制率为76.05%。大蒜乙醇提取物在10% (V / V)剂量下对真菌的抑制作用为100%,第5天对平均菌丝生长的抑制作用较低,从48.72%降至6% (V / V),而生姜乙醇提取物在8% (V / V)剂量下对公众效应的抑制作用较好,在3天内抑制作用为100%。其对菌丝生长的抑制作用较低,平均为76.98% ~ 6% (V / V)。所研究的植物乙醇提取物为传统和工业化农业中植物病原真菌的生物防治开辟了一条途径。
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Efficiency of Ethanolic Extracts of Vegetables for the in Vitro Control of the Phytopathogenic Fungus (Fusarium Verticillioides)
In the present work the efficiency of the ethanolic extracts of Allium sativum, Allium cepa and Zingiber officinale was evaluated on the mycelial growth of the fungus Fusarium verticillioides, said phytopathogen has the power to infect and cause disease in Zea mays crops. The agar dilution technique was used, 5 repetitions were made by extract and dose, which were measured in 3 different times, in addition to the two-factor ANOVA experimental design with a level of significance of α = 0.05. The ethanol extract of Allium sativum showed an inhibition in the mycelial growth of the fungus in 100% with the dose at 13% (V/v), being its lower inhibition in mycelial growth an average of 76.05% with the dose at 11% (V/v) on day 5. The ethanol extract of Allium cepa inhibited the fungus 100% with the dose at 10% (V / v) in its three days of measurement, being its lower inhibition in the average mycelial growth from 48.72% to 6% (V / v) on day 5 and the ethanolic extract of Zingiber officinale which had a better role in terms of the public effect inhibit in 100% with the dose at 8% (V / v) in Its three days of measurement, being its lower inhibition in mycelial growth average of 76.98% to 6% (V / v). The ethanolic extracts of the plants studied give the opening to a way of biological control of phytopathogenic fungi in conventional and industrialized agriculture.
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