三叶Wedelia trilobata根叶银纳米颗粒的制备及抑菌活性研究

Gnana Prakash
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摘要

本研究以三叶菜(Wedelia trilobata)为研究对象,采用溶剂乙醇对其根提取物的体外抗菌活性进行了研究。通过紫外-可见光谱、红外光谱、颗粒大小和zeta电位,纳米颗粒的推导是不可改变的。采用圆盘扩散法研究了三叶线虫介导的银纳米颗粒对大肠杆菌和金黄色葡萄球菌等标准生物的抑菌活性。在紫外可见光谱中,三叶织布草的根和叶分别在450nm和418nm附近有一个特定的吸光度峰,从而证实了其颜色的变化。银纳米粒子的Zeta电位分别为根部0.1和叶片0.4,表明其分散性和稳定性。对革兰氏阳性金黄色葡萄球菌的抑菌活性增强,正常(9.5Mm)、标准(12.3Mm)、对照(11.3Mm)、AgNPs三叶Wedelia三叶虫提取(13.8)的抑菌带直径均从3Mm增加。正常(7.3Mm),标准(19.4Mm),对照(10.5Mm), AgNPs提取(19.8)。对革兰氏阴性大肠杆菌的抑菌活性增强,正常(7.8Mm)、标准(8.5Mm)、对照(10.2Mm)和AgNPs三叶Wedelia提取(14.3)的抑菌带直径均从3Mm增加。AgNPs的正常(13Mm),标准(19.6Mm),对照(15.3Mm)提取(19.8)。本研究表明,所选植物的乙醇根和水叶提取物具有抑制感染菌株扩张的潜力。
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Formulation and Characterization of Anti-Bacterial Activity of Silver Nanoparticles Using Root and Leaf of Wedelia trilobata
In the present study one such species, Wedelia trilobata, has be chosen to review the In vitro antibacterial activity of the root extract by using solvent ethanol for the selected plant Wedelia triolobata. The deduction of nanoparticles used to be unalterable by UV-Visible spectroscopy, FTIR, particle size, and zeta potential. The anti-bacterial activity of Wedelia trilobata mediated synthesis silver nanoparticles was tested by disc diffusion assay against standard organisms like Escherichia coli and staphylococcus aureus bacteria. The formation of silver nanoparticles by Wedelia trilobata was initially demonstrated by color changes confirmed by UV-Visible root exhibited a specific absorbance peak around 450 nm and leaf exhibited a specific absorbance peak around 418nm respectively. The Zeta potential of silver nanoparticles was found to be root 0.1 and leaf 0.4, so it indicates the dispersion and stability. The antibacterial activity against Gram-positive Staphylococcus aureus was increased, which was indicated by an increase in the inhibition zone diameter from 3Mm for normal (9.5Mm), standard (12.3Mm), control (11.3Mm), extraction for AgNPs Wedelia trilobata (13.8). 5Mm for normal (7.3Mm), standard (19.4Mm), control (10.5Mm), extraction for AgNPs Wedelia trilobata (19.8). The antibacterial activity against Gram-negative E.coli was increased, which was indicated by an increase in the inhibition zone diameter from 3Mm for normal (7.8Mm), standard (8.5Mm), control (10.2Mm), extraction for AgNPs Wedelia trilobata (14.3). 5Mm for normal (13Mm), standard (19.6Mm), control (15.3Mm) extraction for AgNPs Wedelia trilobata (19.8). The present investigation revealed that the ethanolic Root and aqueous leaf extracts the chosen plant have potential to suppress the expansion of infective bacterial strains.
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