巴沙鱼皮胶原蛋白的提取及其抗氧化活性

T. Le, L. Lê, Dang Giang Chau Nguyen, Thanh Minh Trần, Thuy-Trang Le, Hoang Luong Ngoc Nguyen, Thi Nhu Nguyen, Thi Van Thi Tran
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引用次数: 0

摘要

鱼类加工业排出的鱼皮是生产胶原蛋白的丰富原料来源。本研究旨在优化从博库蒂巴沙鱼皮中提取胶原蛋白的实验条件。在NaOH 0.1 M、柠檬酸0.05 M脱矿提取、5% H2O2 200 mL的最佳条件下得率最高。通过红外光谱和扫描电镜对胶原蛋白进行了表征,并对胶原蛋白的体外抗氧化活性进行了评价。所得胶原具有明显的总抗氧化活性、ABTS自由基清除活性和DPPH自由基清除活性。
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Collagen from Pangasius bocourti skin: extraction and antioxidant activities
Fish skin discharged from the fish processing industry is an abundant source of raw material for collagen production. This work aims to optimise the experiment conditions for collagen extraction from Pangasius bocourti skin. The highest yield was obtained under the following optimal conditions: NaOH 0.1 M, citric acid 0.05 M for demineralisation and extraction, and 200 mL of 5% H2O2. The collagen was characterised from its IR spectra and SEM images, and the antioxidant activities of collagen were evaluated in vitro. The obtained collagen demonstrated appreciable total antioxidant activity, ABTS radical scavenging activity, and DPPH radical scavenging activity.
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